Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
Open Access
- 3 February 2014
- journal article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 4 (1), 3943
- https://doi.org/10.1038/srep03943
Abstract
Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.This publication has 24 references indexed in Scilit:
- Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNAScientific Reports, 2013
- Efficient nonmeiotic allele introgression in livestock using custom endonucleasesProceedings of the National Academy of Sciences of the United States of America, 2013
- Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafishNucleic Acids Research, 2013
- Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene ExpressionCell, 2013
- Programmable plasmid interference by the CRISPR-Cas system inThermococcus kodakarensisRNA Biology, 2013
- TALENs: a widely applicable technology for targeted genome editingNature Reviews Molecular Cell Biology, 2012
- RNAimmuno: A database of the nonspecific immunological effects of RNA interference and microRNA reagentsRNA, 2012
- Functional Identification of Optimized RNAi Triggers Using a Massively Parallel Sensor AssayMolecular Cell, 2011
- Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigsHuman Molecular Genetics, 2010
- Therapeutic Silencing of MicroRNA-122 in Primates with Chronic Hepatitis C Virus InfectionScience, 2010