Cytokine production by human leukocytes with different expressions of natural antiviral immunity and the effect of antibodies against interferons and TNF-±
Open Access
- 20 March 2007
- journal article
- Published by Walter de Gruyter GmbH in Archivum Immunologiae et Therapiae Experimentalis
- Vol. 55 (2), 111-117
- https://doi.org/10.1007/s00005-007-0013-y
Abstract
Two activities of innate antiviral immunity were studied: the resistance of human peripheral blood mononuclear cells (PMBCs) ex vivo to viral infection and the production of cytokines. Samples of blood were taken from healthy blood donors and from persons with frequent infections of the upper respiratory system. PMBCs were isolated by gradient centrifugation. Vesicular stomatitis virus (VSV) was used as the indicatory virus to infect PMBCs. The cytokines: IFN, TNF, and IL-6 were titrated by biological methods and IL-10 by ELISA. Blood donors were divided for two groups: those with VSV-resistant and those with VSV-sensitive PMBCs and secretion of cytokines by them was compared. The resistant PMBCs produced more cytokines than the sensitive ones. A statistically significant difference, was found only in the case of the IFNs. To examine the contribution of IFNs and TNF in maintaining resistance, leukocytes from both groups were treated with specific anti-cytokine antibodies. The authors’ previous study showed that the elimination of spontaneous IFN-±, IFN-β, IFN-γ, and TNF-± from resistant leukocytes resulted in increased VSV replication This indicates the important role of cytokines. In VSV-sensitive PMBCs, anti-IFN-± showed the opposite effect (decreased virus replication). In the absence of spontaneous IFN-±, disturbances in cytokine production were observed. Complete resistance of PMBC to VSV infection is accompanied by higher cytokine release, The paradoxical effect of anti-IFN-± on virus replication in leukocytes sensitive to viral infection may be attributed to changes in the cytokine profile balance, i.e. high TNF production by VSV-infected leukocytes and a complete reduction of IL-6 production.Keywords
This publication has 27 references indexed in Scilit:
- Mannan-binding lectin activates C3 and the alternative complement pathway without involvement of C2JCI Insight, 2006
- Interferons as pathogenic effectors in autoimmunityImmunological Reviews, 2005
- Toll‐like receptors, endogenous ligands, and systemic autoimmune diseaseImmunological Reviews, 2005
- CD40 signaling regulates innate and adaptive activation of microglia in response to amyloid β‐peptideEuropean Journal of Immunology, 2005
- Innate immunityCurrent Opinion in Immunology, 2003
- Study on Risk Factors for Transplacental Viral Infections; Effect of Bacterial Factors and Double Viral Infections on Virus Replication in Placenta and Amniotic MembranesPlacenta, 2001
- Effect of Proline Rich Polypeptide from Ovine Colostrum on Virus Replication in Human Placenta and Amniotic Membrane at Term; Possible Role of Endogenous Tumour Necrosis Factor αPlacenta, 1999
- Antiviral Nonspecific Immunity of Human Placenta at Term: Possible Role of Endogenous Tumor Necrosis Factors and InterferonsJournal of Interferon & Cytokine Research, 1996
- Production of Cytokines with Antiviral Activity by Endothelial CellsJournal of Interferon & Cytokine Research, 1995
- Antiviral effects of recombinant tumour necrosis factor in vitroNature, 1986