In VitroMethods of Assay of Antioxidants: An Overview

Abstract

With the current upsurge of interest in the function, measurement of efficacy and use of natural antioxidants, testing of antioxidant activity has received much attention. Several methods are being used, both in vitro and in vivo, to determine the antioxidant activity of natural antioxidants. Although, there is a great multiplicity of the methods used for the determination of antioxidant activity, there is no approved standardized in vitro method to evaluate the antioxidants of interest. This article presents a critical review of the various in vitro methods used for the determination of antioxidant activity and their merits and limitations.