Hypercholesterolemia impairs reactive hyperemic vasodilation of 2A but not 3A arterioles in mouse cremaster muscle
Open Access
- 1 July 2005
- journal article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 289 (1), H447-H454
- https://doi.org/10.1152/ajpheart.01298.2004
Abstract
Hypercholesterolemia and atherosclerosis have been associated with changes in the microvasculature, in particular with endothelial dysfunction. In the present study, the impact of atherogenic conditions on arteriolar vasomotor control was determined. Arteriolar [second-order (2A) and third-order (3A) arterioles; diameter range: 9–37 μm] responses during reactive hyperemia (RH) were determined in cremaster muscle of anesthetized mice. C57Bl/6 mice on normal rodent chow were used as controls and high-fat/high-cholesterol (HFC)-fed C57Bl/6 and ApoE3-Leiden mice as hypercholesterolemic mice. The HFC diet resulted in time-dependent increases in plasma cholesterol and triglyceride concentrations ( P < 0.001), which were more pronounced in ApoE3-Leiden mice ( P < 0.001). In control mice, inhibition of nitric oxide (NO) synthesis with Nω-nitro-l-arginine (l-NNA) reduced baseline diameter from 17.9 ± 1.2 to 15.9 ± 1.3 μm ( P < 0.05) and decreased the duration of RH [time to 50% ( t50) of recovery: 23.3 ± 3.6 vs. 12.5 ± 1.3 s ( P = 0.003)]. t50was longer in 2A versus 3A arterioles (33 ± 3 vs. 18 ± 2 s, P < 0.001) and increased with wall shear rate at the beginning of RH in 2A arterioles only. Compared with control mice, RH duration was reduced in 2A arterioles of HFC mice ( t50: 11 ± 2 s, P < 0.001 vs. control) but not affected in 3A vessels. l-NNA did not affect baseline diameter in HFC mice and reduced t50only in “slow” responders ( t50≥ 10 s). It is concluded that hypercholesterolemia results in an impairment of NO-mediated vasomotor control in 2A but not 3A arterioles during dynamic changes of perfusion like RH. 2A arterioles likely therefore represent the functional locus of endothelial dysfunction during atherogenic conditions.Keywords
This publication has 35 references indexed in Scilit:
- Hypercholesterolemia in Minipigs Impairs Left Ventricular Response to StressCirculation, 2002
- Nitric Oxide Opposes Myogenic Pressure Responses Predominantly in Large Arterioles In VivoHypertension, 1998
- The microcirculation in atherogenesisCardiovascular Research, 1996
- Quantitative Assessment of Aortic Atherosclerosis in APOE*3 Leiden Transgenic Mice and Its Relationship to Serum Cholesterol ExposureArteriosclerosis, Thrombosis, and Vascular Biology, 1996
- Modulation of very low density lipoprotein production and clearance contributes to age- and gender- dependent hyperlipoproteinemia in apolipoprotein E3-Leiden transgenic mice.JCI Insight, 1996
- Regulation of coronary blood flow: coordination of heterogeneous control mechanisms in vascular microdomainsCardiovascular Research, 1995
- The antioxidant butylated hydroxytoluene prevents early cholesterol-induced microcirculatory changes in rabbits.JCI Insight, 1994
- Diet-induced hyperlipoproteinemia and atherosclerosis in apolipoprotein E3-Leiden transgenic mice.JCI Insight, 1994
- Comparison of early microcirculatory and aortic changes in hypercholesterolemic rats.Arteriosclerosis and Thrombosis: A Journal of Vascular Biology, 1991
- Determination of volumetric flow in capillary tubes using an optical Doppler velocimeterMicrovascular Research, 1987