Preferential effects of nicotine and 4-(N-methyl- N-nitrosamino)-1-(3-pyridyl)-1-butanone on mitochondrial glutathione S-transferase a4-4 induction and increased oxidative stress in the rat brain
- 1 October 1998
- journal article
- Published by Elsevier BV in Biochemical Pharmacology
- Vol. 56 (7), 831-839
- https://doi.org/10.1016/s0006-2952(98)00228-7
Abstract
We have investigated the in vivo effects of the tobacco-specific toxins nicotine and 4-( N -methyl- N -nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) on antioxidant defense systems in the mitochondrial, microsomal, and cytosolic compartments of rat brain, lung, and liver. Nicotine induced maximum oxidative stress in brain mitochondria, as seen from a 1.9-fold ( P < 0.001) increase in thiobarbituric acid-reactive substance (TBARS) and a 2-fold ( P < 0.001) increase in glutathione S -transferase (GST) A4-4 (also referred to as rGST 8-8) activities. These changes were accompanied by a 25–40% increase in reactive oxygen species and a 20–30% decrease in alcohol dehydrogenase activities. The 4-( N -methyl- N -nitrosamino)-1-(3-pyridyl)-l-butanone-induced oxidative damage was apparent in the microsomal fraction of brain, lung, and liver, and it also increased 4-hydroxynonenal specific GST A4-4 activity in the brain and lung mitochondrial matrix fraction. The levels of microsomal thiobarbituric acid reactive substance, cytochrome P4502E1 activity, and reactive oxygen species were also increased significantly ( P < 0.001) in all tissues. Both of these toxins induced the level of GST A4-4 mRNA in the brain, while they caused a marked reduction in the liver GST A4-4 mRNA pool. Additionally, the brain mitochondrial matrix showed a markedly higher level of 4-hydroxynonenal specific GST activity and mGST A4-4 antibody-reactive protein than did the cytosolic fraction. In conclusion, the present study provides evidence for the occurrence of GST A4-4 enzyme activity in mammalian mitochondria, in addition to demonstrating that both mitochondria and microsomes are intracellular targets for nicotine- and NNK-induced organ toxicity.Keywords
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