Stabilization of Oat Leaf Protoplasts through Polyamine-mediated Inhibition of Senescence

Abstract
Protoplasts isolated from Avena sativa L. leaves undergo progressive senescence when incubated aseptically in 0.6 m mannitol with or without added nutrients. This senescence is manifested by morphological deterioration and ultimate lysis of protoplasts, by a decrease in incorporation of [3H]uridine and [3H]leucine into macromolecules, and by a sharp increase in ribonuclease activity.