Uniparental expression of PolIV-dependent siRNAs in developing endosperm of Arabidopsis
- 3 June 2009
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 460 (7252), 283-286
- https://doi.org/10.1038/nature08084
Abstract
Most eukaryotes produce small RNA (sRNA) mediators of gene silencing that bind to Argonaute proteins and guide them, by base pairing, to an RNA target. MicroRNAs (miRNAs) that normally target messenger RNAs for degradation or translational arrest are the best-understood class of sRNAs. However, in Arabidopsis thaliana flowers, miRNAs account for only 5% of the sRNA mass and less than 0.1% of the sequence complexity. The remaining sRNAs form a complex population of more than 100,000 different small interfering RNAs (siRNAs) transcribed from thousands of loci. The biogenesis of most of the siRNAs in Arabidopsis are dependent on RNA polymerase IV (PolIV), a homologue of DNA-dependent RNA polymerase II. A subset of these PolIV-dependent (p4)-siRNAs are involved in stress responses, and others are associated with epigenetic modifications to DNA or chromatin; however, the biological role is not known for most of them. Here we show that the predominant phase of p4-siRNA accumulation is initiated in the maternal gametophyte and continues during seed development. Expression of p4-siRNAs in developing endosperm is specifically from maternal chromosomes. Our results provide the first evidence for a link between genomic imprinting and RNA silencing in plants.Keywords
This publication has 21 references indexed in Scilit:
- PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesisProceedings of the National Academy of Sciences of the United States of America, 2008
- Role of RNA polymerase IV in plant small RNA metabolismProceedings of the National Academy of Sciences of the United States of America, 2007
- Polycomb Group Complexes Self-Regulate Imprinting of the Polycomb Group Gene MEDEA in ArabidopsisCurrent Biology, 2006
- Atypical RNA polymerase subunits required for RNA-directed DNA methylationNature Genetics, 2005
- The Small RNA Profile during Drosophila melanogaster DevelopmentDevelopmental Cell, 2003
- ThePolycomb-group protein MEDEA regulates seed development by controlling expression of the MADS-box genePHERES1Genes & Development, 2003
- MicroRNAs in plantsGenes & Development, 2002
- Delayed activation of the paternal genome during seed developmentNature, 2000
- Imprinting of the MEDEA Polycomb Gene in the Arabidopsis EndospermPlant Cell, 1999
- Developmental abnormalities and epimutations associated with DNA hypomethylation mutations.Proceedings of the National Academy of Sciences of the United States of America, 1996