RNA-Seq analysis of Citrus reticulata in the early stages of Xylella fastidiosa infection reveals auxin-related genes as a defense response
Open Access
- 3 October 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Genomics
- Vol. 14 (1), 676
- https://doi.org/10.1186/1471-2164-14-676
Abstract
Background: Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (Citrus sinensis L. Osb). On the other hand, among the Citrus genus there are different sources of resistance against X. fastidiosa. For these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. To assess these genes we made use of mandarin (C. reticulata Blanco) that is known to be resistant to CVC and shares agronomical characteristics with sweet orange. Thus, we investigated the gene expression in Ponkan mandarin at one day after infection with X. fastidiosa, using RNA-seq. A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange. Results: Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later. Among the induced transcripts, we identified genes encoding proteins similar to Pattern Recognition Receptors. Furthermore, many genes involved in secondary metabolism, biosynthesis and cell wall modification were upregulated as well as in synthesis of abscisic acid, jasmonic acid and auxin. Conclusions: This work demonstrated that the defense response to the perception of bacteria involves cell wall modification and activation of hormone pathways, which probably lead to the induction of other defense-related genes. We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.This publication has 57 references indexed in Scilit:
- Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiationNature Biotechnology, 2010
- Cell Wall-Degrading Enzymes Enlarge the Pore Size of Intervessel Pit Membranes in Healthy andXylella fastidiosa-Infected GrapevinesPlant Physiology, 2010
- Stem Cell Signaling in Arabidopsis Requires CRN to Localize CLV2 to the Plasma MembranePlant Physiology, 2009
- Functional Analyses of the CLAVATA2-Like Proteins and Their Domains That Contribute to CLAVATA2 SpecificityPlant Physiology, 2009
- Recent Advances in PAMP-Triggered Immunity against Bacteria: Pattern Recognition Receptors Watch over and Raise the AlarmPlant Physiology, 2009
- Evolutionary History and Stress Regulation of Plant Receptor-Like Kinase/Pelle GenesPlant Physiology, 2009
- TopHat: discovering splice junctions with RNA-SeqBioinformatics, 2009
- Activation of the Indole-3-Acetic Acid–Amido Synthetase GH3-8 Suppresses Expansin Expression and Promotes Salicylate- and Jasmonate-Independent Basal Immunity in RicePlant Cell, 2008
- The plant immune systemNature, 2006
- Bacterial elicitation and evasion of plant innate immunityNature Reviews Molecular Cell Biology, 2006