Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation
- 12 March 2009
- journal article
- Published by Wiley in Reproduction in Domestic Animals
- Vol. 44 (2), 212-220
- https://doi.org/10.1111/j.1439-0531.2007.01032.x
Abstract
We have approached the problem of refrigerated storage of epididymal sperm samples from red deer by comparing three options: storing the genital (testicles within the scrotum), diluting the semen in extender or diluting the semen in extender supplemented with an anti-oxidant. Twenty-nine pairs of testes were collected. Spermatozoa from one of each of the pairs were immediately recovered, and diluted to 400 × 106 sperm/ml in Tris-citrate-fructose with 20% egg yolk. Control group was stored as such, and Anti-oxidant group was supplemented with 0.8 mm vitamin C. The remaining epididymides and the diluted samples were stored at 5°C and spermatozoa were analysed at 0, 24, 96 and 192 h for: motility [computer-assisted semen analysis (CASA)], acrosomal integrity, sperm viability (eosine/nigrosine staining), normal tails and chromatin status [sperm chromatin structure assay (SCSA)]. In general, seminal quality decreased with storage time. Vitamin C supported progressive motility better at 24 h (median 42% vs 23% Control and 15% epididymis), reduced the incidence of tail abnormalities and protected chromatin. Storing the semen in the epididymis slowed down motility loss, but slightly increased the occurrence of tail abnormalities and viability was lower at 192 h. However, regarding chromatin status, sperm stored in the epididymis was protected similarly to those diluted in the medium supplemented with vitamin C. Although the differences between the three groups were small, there were some advantages in supplementing the extender with vitamin C. Besides, refrigerating the epididymis may be a good option when immediate processing is not available.Keywords
This publication has 55 references indexed in Scilit:
- Sperm Characteristics and DNA Integrity of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa Frozen in the Presence of Enzymatic and Nonenzymatic AntioxidantsJournal of Andrology, 2007
- Influence of Various Permeating Cryoprotectants on Freezability of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa: Effects of Concentration and Temperature of AdditionJournal of Andrology, 2006
- Effects of Egg Yolk and Cooling Rate on the Survival of Refrigerated Red Deer (Cervus elaphus hispanicus) Epididymal SpermatozoaReproduction in Domestic Animals, 2006
- Effect of Antioxidant Intake on Sperm Chromatin Stability in Healthy Nonsmoking MenJournal of Andrology, 2005
- Storage of red deer epididymides for four days at 5°C: Effects on sperm motility, viability, and morphological integrityJournal of Experimental Zoology Part A: Comparative Experimental Biology, 2003
- Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile menFertility and Sterility, 1997
- Liquid storage of ram semen in the absence or presence of some antioxidantsReproduction, Fertility and Development, 1996
- Oestrous synchronization, semen preservation and artificial insemination in the Mohor gazelle (Gazella dama mhorr) for the establishment of a genome resource bank programmeReproduction, Fertility and Development, 1996
- Lipids and calcium uptake of sperm in relation to cold shock and preservation: a reviewReproduction, Fertility and Development, 1993
- Vitamin C—The primate fertility factor?Medical Hypotheses, 1992