Peptide Biomarkers as Evidence of Perchlorate Biodegradation
- 1 February 2011
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (3), 810-820
- https://doi.org/10.1128/aem.01323-10
Abstract
Perchlorate is a known health hazard for humans, fish, and other species. Therefore, it is important to assess the response of an ecosystem exposed to perchlorate contamination. The data reported here show that a liquid chromatography-mass spectrometry-based proteomics approach for the detection of perchlorate-reducing enzymes can be used to measure the ability of microorganisms to degrade perchlorate, including determining the current perchlorate degradation status. Signature peptides derived from chlorite dismutase (CD) and perchlorate reductase can be used as biomarkers of perchlorate presence and biodegradation. Four peptides each derived from CD and perchlorate reductase subunit A (PcrA) and seven peptides derived from perchlorate reductase subunit B (PcrB) were identified as signature biomarkers for perchlorate degradation, as these sequences are conserved in the majority of the pure and mixed perchlorate-degrading microbial cultures examined. However, chlorite dismutase signature biomarker peptides from Dechloromonas agitata CKB were found to be different from those in other cultures used and should also be included with selected CD biomarkers. The combination of these peptides derived from the two enzymes represents a promising perchlorate presence/biodegradation biomarker system. The biomarker peptides were detected at perchlorate concentrations as low as 0.1 mM and at different time points both in pure cultures and within perchlorate-reducing environmental enrichment consortia. The peptide biomarkers were also detected in the simultaneous presence of perchlorate and an alternate electron acceptor, nitrate. We believe that this technique can be useful for monitoring bioremediation processes for other anthropogenic environmental contaminants with known metabolic pathways.Keywords
This publication has 27 references indexed in Scilit:
- Proteomic detection of proteins involved in perchlorate and chlorate metabolismBiodegradation, 2009
- Chemical and Steady-State Kinetic Analyses of a Heterologously Expressed Heme Dependent Chlorite DismutaseBiochemistry, 2008
- Quantitative Detection of Perchlorate-Reducing Bacteria by Real-Time PCR Targeting the Perchlorate Reductase GeneApplied and Environmental Microbiology, 2008
- Characterization of the proteins of bacterial strain isolated from contaminated site involved in heavy metal resistance—A proteomic approachJournal of Biotechnology, 2007
- Environmental proteomics and metallomicsProteomics, 2006
- Metabolic Primers for Detection of (Per)chlorate-Reducing Bacteria in the Environment and Phylogenetic Analysis of cld Gene SequencesApplied and Environmental Microbiology, 2004
- Changes in protein expression profiles in bivalve molluscs (Chamaelea gallina) exposed to four model environmental pollutantsProteomics, 2003
- Universal Immunoprobe for (Per)Chlorate-Reducing BacteriaApplied and Environmental Microbiology, 2002
- Biomarkers as biological indicators of xenobiotic exposureJournal of Applied Toxicology, 2001
- A Proteome Analysis of the Cadmium Response in Saccharomyces cerevisiaePublished by Elsevier BV ,2001