Enzymatic Synthesis of Carbohydrate Derivatives Using β-Glycosidase ofSulfolobus Solfataricus
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis Ltd in Biocatalysis
- Vol. 10 (1-4), 195-210
- https://doi.org/10.3109/10242429409065229
Abstract
Enzymatic synthesis of different β-D-glycosides was obtained using as biocatalyst immobilized cells, crude homogenate, and homogeneous native and recombinat β-glycosidase activity of the thermophilic archaeon Sulfolobus solfataricus. In particular our investigation was concerned with the selectivity in the glycosylation of hydroxybenzyl alcohols, salicin, 1,2-propanediol, and more complex polyols as well as the use of immobilized cells for the synthesis of hexyl-β-D-glucoside. The aromatic glucosides obtained by enzyme-catalyzed transglucosylation were used for kinetic studies of purified Sulfolobus solfataricus enzyme in the hydrolysis reaction.Keywords
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