Development of a mariner -Based Transposon for Use in Sorangium cellulosum
Open Access
- 1 October 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (10), 6299-6301
- https://doi.org/10.1128/aem.69.10.6299-6301.2003
Abstract
In order to generate marked insertions in the myxobacterium Sorangium cellulosum , a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.Keywords
This publication has 23 references indexed in Scilit:
- Identification of genes required for adventurous gliding motility in Myxococcus xanthus with the transposable element marinerMolecular Microbiology, 2003
- Characterisation, genome size and genetic manipulation of the myxobacterium Sorangium cellulosum So ce56Archiv für Mikrobiologie, 2002
- Random Transposon Mutagenesis of Campylobacter jejuniInfection and Immunity, 2000
- In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner -family transposable element Himar1Proceedings of the National Academy of Sciences of the United States of America, 2000
- Myxobacteria as Producers of Secondary MetabolitesPublished by Springer Science and Business Media LLC ,2000
- Tolerance and Specificity of Polyketide SynthasesAnnual Review of Biochemistry, 1999
- Tn 5 /IS 50 target recognitionProceedings of the National Academy of Sciences, 1998
- Transfer of mobilizable plasmids to Sorangium cellosum and evidence for their integration into the chromosomePlasmid, 1992
- MOLECULAR GENETICS OF POLYKETIDES AND ITS COMPARISON TO FATTY ACID BIOSYNTHESISAnnual Review of Genetics, 1990
- Promoters largely determine the efficiency of repressor action.Proceedings of the National Academy of Sciences of the United States of America, 1988