ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation
- 1 May 2007
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Pain
- Vol. 129 (1), 102-112
- https://doi.org/10.1016/j.pain.2006.09.038
Abstract
Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat, hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3-/- and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3-/- mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/+ mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3-/- mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3-/- mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult.Keywords
This publication has 42 references indexed in Scilit:
- The Mechanosensitivity of Mouse Colon Afferent Fibers and Their Sensitization by Inflammatory Mediators Require Transient Receptor Potential Vanilloid 1 and Acid-Sensing Ion Channel 3Journal of Neuroscience, 2005
- Involvement of transient receptor potential vanilloid 1 in the vascular and hyperalgesic components of joint inflammationArthritis & Rheumatism, 2005
- Factors involved in strain-induced injury in skeletal muscles and outcomes of prolonged exposuresJournal of Electromyography and Kinesiology, 2004
- Rapid method for construction of recombinant HSV gene transfer vectorsGene Therapy, 1997
- A proton-gated cation channel involved in acid-sensingNature, 1997
- Myositis-induced functional reorganisation of the rat dorsal horn: effects of spinal superfusion with antagonists to neurokinin and glutamate receptorsPain, 1997
- unc-8, a DEG/ENaC Family Member, Encodes a Subunit of a Candidate Mechanically Gated Channel That Modulates C. elegans LocomotionNeuron, 1997
- Gene interactions affecting mechanosensory transduction in Caenorhabditis elegansNature, 1994
- The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degenerationNature, 1991
- Histological and neurophysiological changes induced by carrageenan in skeletal muscle of cat and ratInflammation Research, 1988