A Rapid Bioassay for Screening Rhizosphere Microorganisms for Their Ability to Induce Systemic Resistance
Open Access
- 1 April 2000
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 90 (4), 327-332
- https://doi.org/10.1094/phyto.2000.90.4.327
Abstract
We developed a rapid and miniaturized bioassay for screening large numbers of rhizosphere microorganisms for their ability to induce systemic resistance to bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae. In this bioassay, Pantoea agglomerans strain E278Ar controlled symptoms of disease as effectively as 2,6-dichloroisonicotinic acid when applied to the roots of seedlings produced in growth pouches in a soilless system. E278Ar essentially did not migrate from seedling roots to the foliage. This suggests that induction of systemic resistance could best explain the observed reduction in disease severity. Three mini-Tn5Km-induced mutants of strain E278Ar were isolated that had lost the ability to induce resistance. The bioassay also was used to demonstrate that the fungal biocontrol agent Trichoderma hamatum strain 382 induces systemic resistance in radish. The bioassay required only 14 to 18 days from seeding until rating for disease severity, which is 10 to 14 days less than earlier bioassays.Keywords
This publication has 41 references indexed in Scilit:
- Salicylic Acid Biosynthetic Genes Expressed in Pseudomonas fluorescens Strain P3 Improve the Induction of Systemic Resistance in Tobacco Against Tobacco Necrosis VirusPhytopathology®, 1998
- A New Pathotype of Xanthomonas campestris pv. armoraciae That Causes Bacterial Leaf Spot of RadishPlant Disease, 1997
- The Chemistry of Benzothiadiazole Plant ActivatorsPesticide Science, 1997
- Genetic Dissection of Acquired Resistance to DiseasePlant Physiology, 1997
- Salicylic Acid Mediates Elicitin-lnduced Systemic Acquired Resistance, but Not Necrosis in TobaccoMolecular Plant-Microbe Interactions®, 1996
- Induction of systemic resistance byPseudomonas fluorescens in radish cultivars differing in susceptibility to fusarium wilt, using a novel bioassayEuropean Journal of Plant Pathology, 1995
- Salicylic Acid Induced Resistance toErwinia carotovorasubsp,carotovorain TobaccoMolecular Plant-Microbe Interactions®, 1994
- Research NotesA New Multigene Family Inducible by Tobacco Mosaic Virus or Salicylic Acid in TobaccoMolecular Plant-Microbe Interactions®, 1992
- Induced Systemic Resistance to Blue Mold: Early Induction and Accumulation ofβ-1,3-Glucanases, Chitinases, and Other Pathogenesis-Related Proteins (b-Proteins) in Immunized TobaccoPhytopathology®, 1989
- Persistence of Induced Systemic Resistance to Blue Mold in Tobacco Plants Derived Via Tissue CulturePhytopathology®, 1987