The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility
Open Access
- 15 September 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Journal of Assisted Reproduction and Genetics
- Vol. 28 (1), 49-54
- https://doi.org/10.1007/s10815-010-9479-3
Abstract
Patients with unexplained infertility may have fertilization problems. Split fertilization (ICSI and conventional IVF on sibling oocytes) is often used to avoid poor fertilization. Our aim was to assess the ability of hyaluronic acid binding (HA-binding) assay to predict spontaneous fertilization during IVF. Prospective, blinded, controlled trial. Patients undergoing their first IVF cycle for unexplained infertility were eligible. Split fertilization was used. IVF and ICSI fertilization rates and embryo development based on 3 HA-binding cut-offs (< 60%; 60-80%; >80%) were compared. ICSI fertilization was higher than IVF, but none of the HA-binding cut-off levels predicted those cases where IVF was less effective, therefore ICSI only would have lead to improved outcome. Embryo development and morphology were similar in all cut-off groups. HA-binding did not predict spontaneous fertilization in patients with unexplained infertility undergoing IVF treatment. When it was used for "screening" it did not help to select the method of fertilization.Keywords
This publication has 26 references indexed in Scilit:
- Sperm–hyaluronan-binding assay: clinical value in conventional IVF under Italian lawReproductive BioMedicine Online, 2009
- Unexplained infertility: Does it really exist?Human Reproduction, 2006
- Relationship between human sperm-hyaluronan binding assay and fertilization rate in conventional in vitro fertilizationHuman Reproduction, 2006
- Conventional in vitro fertilization versus intracytoplasmic sperm injection in patients with borderline semen: a randomized study using sibling oocytesFertility and Sterility, 2006
- Sperm function tests and fertilityInternational Journal of Andrology, 2006
- Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidiesFertility and Sterility, 2005
- Further considerations on natural or mild hyperstimulation cycles for intrauterine insemination treatment: effects on pregnancy and multiple pregnancy ratesHuman Reproduction, 2005
- Efficacy of Superovulation and Intrauterine Insemination in the Treatment of InfertilityThe New England Journal of Medicine, 1999
- Treatment of Endometriosis-Associated InfertilitySeminars in Reproductive Medicine, 1997
- Intrauterine insemination: evaluation of the results according to the woman's age, sperm quality, total sperm count per insemination and life table analysisHuman Reproduction, 1996