Impaired endochondral ossification and angiogenesis in mice deficient in membrane-type matrix metalloproteinase I
Open Access
- 28 March 2000
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 97 (8), 4052-4057
- https://doi.org/10.1073/pnas.060037197
Abstract
Membrane-type matrix metalloproteinase I (MT1-MMP)-deficient mice were found to have severe defects in skeletal development and angiogenesis. The craniofacial, axial, and appendicular skeletons were severely affected, leading to a short and domed skull, marked deceleration of postnatal growth, and death by 3 wk of age. Shortening of bones is a consequence of decreased chondrocyte proliferation in the proliferative zone of the growth plates. Defective vascular invasion of cartilage leads to enlargement of hypertrophic zones of growth plates and delayed formation of secondary ossification centers in long bones. In an in vivo corneal angiogenesis assay, null mice did not have angiogenic response to implanted FGF-2, suggesting that the defect in angiogenesis is not restricted to cartilage alone. In tissues from null mice, activation of latent matrix metalloproteinase 2 was deficient, suggesting that MT1-MMP is essential for its activation in vivo.Keywords
This publication has 28 references indexed in Scilit:
- In Vivo Evidence That the Stromelysin-3 Metalloproteinase Contributes in a Paracrine Manner to Epithelial Cell MalignancyThe Journal of cell biology, 1998
- Requirement for Macrophage Elastase for Cigarette Smoke-Induced Emphysema in MiceScience, 1997
- Unaltered Secretion of β-Amyloid Precursor Protein in Gelatinase A (Matrix Metalloproteinase 2)-deficient MiceOnline Journal of Public Health Informatics, 1997
- Intestinal tumorigenesis is suppressed in mice lacking the metalloproteinase matrilysinProceedings of the National Academy of Sciences of the United States of America, 1997
- Membrane Type 1 Matrix Metalloproteinase Digests Interstitial Collagens and Other Extracellular Matrix MacromoleculesOnline Journal of Public Health Informatics, 1997
- Cellular Mechanisms for Human Procollagenase-3 (MMP-13) ActivationJournal of Biological Chemistry, 1996
- Identification of the Second Membrane-type Matrix Metalloproteinase (MT-MMP-2) Gene from a Human Placenta cDNA LibraryOnline Journal of Public Health Informatics, 1995
- cDNA Sequence and mRNA Tissue Distribution of a Novel Human Matrix Metalloproteinase with a Potential Transmembrane SegmentJBIC Journal of Biological Inorganic Chemistry, 1995
- A matrix metalloproteinase expressed on the surface of invasive tumour cellsNature, 1994
- Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridizationHistochemistry and Cell Biology, 1992