Callus and suspension cultures were established from shoot tips of Crataegus monogyna L. in Murashige and Skoog (MS) medium and a modified MS medium. The flavonoid glycosides vitexin, vitexin 2-O-rhamnoside, rutin, and hyperoside, as well as the aglycones apigenin, luteolin, and quercetin, could be detected by TLC and HPLC in, however, varying amounts. Additionally, the contents of procyanidins were determined. The ratio between the contents of flavonoids and procyanidins is discussed.