Optimisation of enzymatic digestion and validation of specimen preservation methods for the analysis of ingested microplastics
- 27 September 2016
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Analytical Methods
- Vol. 9 (9), 1437-1445
- https://doi.org/10.1039/c6ay02343f
Abstract
Microplastics are considered to be a widespread environmental contaminant. Due to their small size microplastics have the potential to be ingested by a range of aquatic organisms which mistake them for a food source and can suffer adverse impacts as a result. Development of standardised methods is imperative to provide reliable and meaningful data when analysing microplastic ingestion by marine fauna. A range of proteolytic digestive enzymes (trypsin, papain and collagenase) were tested to establish optimum digestion efficacy of biological samples and assess the effects of enzymes on microplastics; additionally the applicability of freezing and formaldehyde followed by ethanol as specimen preservation techniques for microplastic research was investigated. Of the enzymes investigated, trypsin yielded the greatest digestive efficacy based on weight reduction (88% ± 2.52 S.D.) at the lowest concentration (0.3125%) with no observed impacts on microplastics. Enumeration of microplastics from wild collected Mytilus edulis revealed mean numbers of 1.05 ± 0.66 S.D. (minimum) to 4.44 ± 3.03 S.D. (maximum) microplastic particles per g wet weight mussel tissue depending on location. There was no significant difference based on preservation method on the quantification of ingested microplastics and no detrimental impacts were observed on the microplastics directly. Enzymatic digestion using trypsin therefore provides a suitable, time and cost effective method to extract microplastics from M. edulis. Furthermore the preservation methods did not have detrimental effects on microplastics, serving to highlight the suitability of biological samples preserved either way for future inquiries into ingested microplastics.Keywords
Funding Information
- University of the Highlands and Islands
This publication has 43 references indexed in Scilit:
- Microplastics in the OceanArchives of Environmental Contamination and Toxicology, 2015
- The impact of debris on marine lifeMarine Pollution Bulletin, 2015
- The physical impacts of microplastics on marine organisms: A reviewEnvironmental Pollution, 2013
- Microplastic Ingestion by ZooplanktonEnvironmental Science & Technology, 2013
- Occurrence of microplastics in the gastrointestinal tract of pelagic and demersal fish from the English ChannelMarine Pollution Bulletin, 2012
- Plastics in the Marine Environment: The Dark Side of a Modern GiftReviews of Environmental Contamination and Toxicology, 2012
- Microplastics in the marine environmentMarine Pollution Bulletin, 2011
- Plastics, the environment and human health: current consensus and future trendsPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2009
- Deposit- and suspension-feeding sea cucumbers (Echinodermata) ingest plastic fragmentsJournal of Experimental Marine Biology and Ecology, 2009
- Ingested Microscopic Plastic Translocates to the Circulatory System of the Mussel, Mytilus edulis (L.)Environmental Science & Technology, 2008