Correlation of mRNA and protein in complex biological samples
Top Cited Papers
- 20 October 2009
- journal article
- review article
- Published by Wiley in FEBS Letters
- Vol. 583 (24), 3966-3973
- https://doi.org/10.1016/j.febslet.2009.10.036
Abstract
The correlation between mRNA and protein abundances in the cell has been reported to be notoriously poor. Recent technological advances in the quantitative analysis of mRNA and protein species in complex samples allow the detailed analysis of this pathway at the center of biological systems. We give an overview of available methods for the identification and quantification of free and ribosome‐bound mRNA, protein abundances and individual protein turnover rates. We review available literature on the correlation of mRNA and protein abundances and discuss biological and technical parameters influencing the correlation of these central biological molecules.Keywords
Funding Information
- EMBO long-term fellowship
- FPU Fellowship from the Spanish Education Ministry
- European Research Council
- Consolider Programme of the Spanish Education Ministry
This publication has 85 references indexed in Scilit:
- Full Dynamic Range Proteome Analysis of S. cerevisiae by Targeted ProteomicsCell, 2009
- 2D-DIGE: Comparative Proteomics of Cellular Signalling PathwaysMethods in molecular biology (Clifton, N.J.), 2009
- A quantitative comparison of sRNA‐based and protein‐based gene regulationMolecular Systems Biology, 2008
- Capture and analysis of quantitative proteomic dataProteomics, 2007
- Protein stains for proteomic applications:Which, when, why?Proteomics, 2006
- Transcriptional Pulsing of a Developmental GeneCurrent Biology, 2006
- Real-Time Kinetics of Gene Activity in Individual BacteriaCell, 2005
- Current two‐dimensional electrophoresis technology for proteomicsProteomics, 2004
- From Silencing to Gene Expression: Real-Time Analysis in Single CellsCell, 2004
- Mass spectrometry-based proteomicsNature, 2003