Quantitative Response Characteristics of Thermoreceptive and Nociceptive Lamina I Spinothalamic Neurons in the Cat

Abstract

The physiological characteristics of antidromically identified lamina I spinothalamic (STT) neurons in the lumbosacral spinal cord were examined using quantitative thermal and mechanical stimuli in barbiturate-anesthetized cats. Cells belonging to the three main recognized classes were included based on categorization with natural cutaneous stimulation of the hindpaw: nociceptive-specific (NS), polymodal nociceptive (HPC), or thermoreceptive-specific (COOL) cells. The mean central conduction latencies of these classes differed significantly; NS = 130.8 ± 55.5 (SD) ms ( n= 100), HPC = 72.1 ± 28.0 ms ( n = 128), and COOL = 58.6 ± 25.3 ms ( n = 136), which correspond to conduction velocities of 2.5, 4.6, and 5.6 m/s. Based on recordings made prior to any noxious stimulation, the mean spontaneous discharge rates of these classes also differed: NS = 0.5 ± 0.7 imp/s ( n = 47), HPC = 0.9 ± 0.7 imp/s ( n = 59), and COOL = 3.3 ± 2.6 imp/s ( n = 107). Standard, quantitative, thermal stimulus sequences applied with a Peltier thermode were used to characterize the stimulus-response functions of 76 COOL cells, 47 HPC cells, and 37 NS cells. The COOL cells showed a very linear output from 34°C down to ∼15°C and a maintained plateau thereafter. The HPC cells showed a fairly linear but accelerating response to cold below a median threshold of ∼24°C and down to 9°C (measured at the skin-thermode interface with a thermode temperature of 2°C). The HPC cells and the NS cells both showed rapidly increasing, sigmoidal response functions to noxious heat with a fairly linear response between 45 and 53°C, but they had significantly different thresholds; half of the HPC cells were activated at ∼45.5°C and half of the NS cells at ∼43°C. The 20 HPC lamina I STT cells and 10 NS cells tested with quantitative pinch stimuli showed fairly linear responses above a threshold of ∼130 g/mm² for HPC cells and a threshold of ∼100 g/mm² for NS cells. All of these response functions compare well (across species) with the available data on the characteristics of thermoreceptive and nociceptive primary afferent fibers and the appropriate psychophysics in humans. Together these results support the concept that these classes of lamina I STT cells provide discrete sensory channels for the sensations of temperature and pain.