Identification and Characterization of a Novel Human Collectin CL‐K1
Open Access
- 1 December 2006
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 50 (12), 1001-1013
- https://doi.org/10.1111/j.1348-0421.2006.tb03868.x
Abstract
Collectins are a family of C‐type lectins with two characteristic structures, collagen like domains and carbohydrate recognition domains. They recognize carbohydrate antigens on microorganisms and act as host‐defense. Here we report the cloning and characterization of a novel collectin CL‐K1. RT‐PCR analyses showed CL‐K1 mRNA is present in all organs. The deduced amino acid sequence and the data from immunostaining of CL‐K1 cDNA expressing CHO cells revealed that CL‐K1 is expressed as a secreted protein. CL‐K1 is found in blood by immunoblotting and partial amino acid analyses. CL‐K1 showed Ca2+‐dependent sugar binding activity of fucose and weakly mannose but not N‐acetyl‐galactosamine, N‐acetyl‐glucosamine, or maltose, though mannose‐binding lectin (MBL) containing similar amino acid motif. CL‐K1 can recognize specially several bacterial saccharides due to specific sugar‐binding character. Elucidation of the role of two ancestor collectins of CL‐K1 and CL‐L1 could lead to see the biological function of collectin family.Keywords
This publication has 32 references indexed in Scilit:
- The Membrane-type Collectin CL-P1 Is a Scavenger Receptor on Vascular Endothelial CellsOnline Journal of Public Health Informatics, 2001
- Chemokines: A New Classification System and Their Role in ImmunityImmunity, 2000
- Molecular and biological characterization of rabbit mannan-binding proteinGlycobiology, 1998
- Structure of a C-type mannose-binding protein complexed with an oligosaccharideNature, 1992
- Engineering galactose-binding activity into a C-type mannose-binding proteinNature, 1992
- Isolation and characterization of conglutinin as an influenza A virus inhibitorBiochemical and Biophysical Research Communications, 1992
- The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10.The Journal of Experimental Medicine, 1989
- Bovine conglutinin is a collagen-like proteinBiochemistry, 1984
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Isolation and characterization of a mannan-binding protein from rabbit serumBiochemical and Biophysical Research Communications, 1980