Functional expression of indoleamine 2,3-dioxygenase by murine CD8α+ dendritic cells
Open Access
- 1 January 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 14 (1), 65-68
- https://doi.org/10.1093/intimm/14.1.65
Abstract
Immunoregulatory antigen-presenting cells (APC) play an important role in maintaining T cell homeostasis and self-tolerance. In particular, recent evidence demonstrates a role for inhibition of T cell proliferation by macrophage tryptophan catabolism involving the activity of the enzyme indoleamine 2,3-dioxygenase (IDO). Dendritic cells (DC) have also been shown to exert immunoregulatory effects mediated by tryptophan catabolism and to cause T cell apoptosis. In the present study, we have comparatively analyzed the expression of IDO activity by murine macrophages and splenic DC. By means of PCR, Western blotting and measurements of enzyme functional activity, we obtained evidence that, different from macrophages, DC constitutively express IDO. Following activation by IFN-γ, the latter cells, in particular the CD8α+ subset, exhibit high functional activity and, unlike macrophages, mediate apoptosis of Th cells in vitro. Therefore, in the mouse, CD8α+ DC may be unique APC capable of fully expressing the IDO mechanism functionally.Keywords
This publication has 18 references indexed in Scilit:
- IL-6 Inhibits the Tolerogenic Function of CD8α+ Dendritic Cells Expressing Indoleamine 2,3-DioxygenasePublished by The American Association of Immunologists ,2001
- Positive Regulatory Role of IL-12 in Macrophages and Modulation by IFN-γPublished by The American Association of Immunologists ,2001
- Regulation of prostaglandin synthesis and cell adhesion by a tryptophan catabolizing enzymeBMC Biochemistry, 2001
- CD40 Ligation Ablates the Tolerogenic Potential of Lymphoid Dendritic CellsThe Journal of Immunology, 2001
- Prevention of T cell–driven complement activation and inflammation by tryptophan catabolism during pregnancyNature Immunology, 2001
- IFN-γ Inhibits Presentation of a Tumor/Self Peptide by CD8α− Dendritic Cells Via Potentiation of the CD8α+ SubsetPublished by The American Association of Immunologists ,2000
- Immunology at the Maternal-Fetal Interface: Lessons for T Cell Tolerance and SuppressionAnnual Review of Immunology, 2000
- Tryptophan catabolism and T-cell tolerance: immunosuppression by starvation?Immunology Today, 1999
- Inhibition of T Cell Proliferation by Macrophage Tryptophan CatabolismThe Journal of Experimental Medicine, 1999
- Prevention of Allogeneic Fetal Rejection by Tryptophan CatabolismScience, 1998