Retaining cell–cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer
- 28 March 2011
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 108 (15), 6235-6240
- https://doi.org/10.1073/pnas.1015938108
Abstract
Primary culture of the cancer cells from patients’ tumors can provide crucial information of individual tumors, yet the technology has not been optimized until now. We developed an innovative culture method for primary colorectal cancer cells, based on the principle that cell–cell contact of cancer cells was maintained throughout the process. When tumor tissue was dissociated into cell clusters, in which cell–cell contact was retained, they rapidly formed spheroids that we termed cancer tissue-originated spheroids (CTOSs). CTOSs of colorectal cancer consisted of highly purified and viable cancer cells, and they were prepared with high efficiency. In immunodeficient mice, CTOSs formed xenograft tumors that retained the features of the parental tumors. Moreover, CTOSs were able to be cultured and expanded in vitro using a 3D culture system and stem cell culture medium. This method allowed evaluation of chemosensitivity and signal pathway activation in cancer cells from individual patients. Easy preparation and culture of pure primary cancer cells provides an innovative platform for studying cancer biology and developing personalized medicine.This publication has 40 references indexed in Scilit:
- The proportion of tumour cells is an independent predictor for survival in colorectal cancer patientsBritish Journal of Cancer, 2010
- It's Diagnostics, StupidCell, 2010
- Newly characterised ex vivo colospheres as a three-dimensional colon cancer cell model of tumour aggressivenessBritish Journal of Cancer, 2009
- E-cadherin directly contributes to PI3K/AKT activation by engaging the PI3K-p85 regulatory subunit to adherens junctions of ovarian carcinoma cellsOncogene, 2009
- Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacityProceedings of the National Academy of Sciences of the United States of America, 2008
- Progestin-Regulated Luminal Cell and Myoepithelial Cell-Specific Responses in Mammary Organoid CultureEndocrinology, 2008
- Identification and expansion of human colon-cancer-initiating cellsNature, 2006
- A human colon cancer cell capable of initiating tumour growth in immunodeficient miceNature, 2006
- The NCI60 human tumour cell line anticancer drug screenNature Reviews Cancer, 2006
- Exploiting the PI3K/AKT Pathway for Cancer Drug DiscoveryNature Reviews Drug Discovery, 2005