The Gene Controlling theIndole Glucosinolate Modifier1Quantitative Trait Locus Alters Indole Glucosinolate Structures and Aphid Resistance inArabidopsis
Open Access
- 1 March 2009
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 21 (3), 985-999
- https://doi.org/10.1105/tpc.108.063115
Abstract
Glucosinolates are defensive secondary compounds that display large structural diversity in Arabidopsis thaliana and related plants. Much attention has been paid to variation in the biosynthesis of Met-derived aliphatic glucosinolates and its ecological consequences, but little is known about the genes that cause qualitative and quantitative differences in Trp-derived indole glucosinolates. We use a combination of quantitative trait locus (QTL) fine-mapping and microarray-based transcript profiling to identify CYP81F2 (At5g57220), encoding a cytochrome P450 monooxygenase, as the gene underlying Indole Glucosinolate Modifier1 (IGM1), a metabolic QTL for the accumulation of two modified indole glucosinolates, 4-hydroxy-indole-3-yl-methyl and 4-methoxy-indole-3-yl-methyl glucosinolate. We verify CYP81F2 function with two SALK T-DNA insertion lines and show that CYP81F2 catalyzes the conversion of indole-3-yl-methyl to 4-hydroxy-indole-3-yl-methyl glucosinolate. We further show that the IGM1 QTL is largely caused by differences in CYP81F2 expression, which results from a combination of cis- and trans-acting expression QTL different from known regulators of indole glucosinolate biosynthesis. Finally, we elucidate a potential function of CYP81F2 in plant–insect interactions and find that CYP81F2 contributes to defense against the green peach aphid (Myzus persicae) but not to resistance against herbivory by larvae from four lepidopteran species.Keywords
This publication has 83 references indexed in Scilit:
- Indole-3-Acetonitrile Production from Indole Glucosinolates Deters Oviposition byPieris rapaePlant Physiology, 2008
- The enzymic and chemically induced decomposition of glucosinolatesPhytochemistry, 2006
- Positive selection driving diversification in plant secondary metabolismProceedings of the National Academy of Sciences of the United States of America, 2006
- The Gene Controlling the Quantitative Trait LocusEPITHIOSPECIFIER MODIFIER1Alters Glucosinolate Hydrolysis and Insect Resistance inArabidopsisPlant Cell, 2006
- Evolutionary dynamics of an Arabidopsis insect resistance quantitative trait locusProceedings of the National Academy of Sciences of the United States of America, 2003
- Trp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3Genes & Development, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Genetic Control of Natural Variation in Arabidopsis Glucosinolate AccumulationPlant Physiology, 2001
- Gene Duplication in the Diversification of Secondary Metabolism: Tandem 2-Oxoglutarate–Dependent Dioxygenases Control Glucosinolate Biosynthesis in ArabidopsisPlant Cell, 2001
- HOOKLESS1, an Ethylene Response Gene, Is Required for Differential Cell Elongation in the Arabidopsis HypocotylCell, 1996