Mutual Regulation of Arabidopsis thaliana Ethylene-responsive Element Binding Protein and a Plant Floral Homeotic Gene, APETALA2
- 4 January 2007
- journal article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 99 (2), 239-244
- https://doi.org/10.1093/aob/mcl265
Abstract
It has previously been shown that Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP) contributed to resistance to abiotic stresses. Interestingly, it has also been reported that expression of ethylene-responsive factor (ERF) genes including AtEBP were regulated by the activity of APETALA2 (AP2), a floral homeotic factor. AP2 is known to regulate expression of several floral-specific homeotic genes such as AGAMOUS. The aim of this study was to clarify the relationship between AP2 and AtEBP in gene expression. Northern blot analysis was performed on ap2 mutants, ethylene-related Arabidopsis mutants and transgenic Arabidopsis plants over-expressing AtEBP, and a T-DNA insertional mutant of AtEBP. Phenotypic analysis of these plants was performed. Expression levels of ERF genes such as AtEBP and AtERF1 were increased in ap2 mutants. Over-expression of AtEBP caused upregulation of AP2 expression in leaves. AP2 expression was suppressed by the null-function of ethylene-insensitive2 (EIN2), although AP2 expression was not affected by ethylene treatment. Loss of AtEBP function slightly reduced the average number of stamens. AP2 and AtEBP are mutually regulated in terms of gene expression. AP2 expression was affected by EIN2 but was not regulated by ethylene treatment.Keywords
This publication has 22 references indexed in Scilit:
- Short-Term Growth Responses to Ethylene in Arabidopsis Seedlings Are EIN3/EIL1 IndependentPlant Physiology, 2004
- A MicroRNA as a Translational Repressor of APETALA2 in Arabidopsis Flower DevelopmentScience, 2004
- The Tomato Transcription Factor Pti4 Regulates Defense-Related Gene Expression via GCC Box and Non-GCC BoxcisElements[W]Plant Cell, 2003
- Redundant Enhancers Mediate Transcriptional Repression of AGAMOUS by APETALA2Developmental Biology, 1999
- Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding proteinProceedings of the National Academy of Sciences of the United States of America, 1997
- The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2.Plant Cell, 1996
- Control of Arabidopsis flower and seed development by the homeotic gene APETALA2.Plant Cell, 1994
- The ABCs of floral homeotic genesCell, 1994
- CTR1, a negative regulator of the ethylene response pathway in arabidopsis, encodes a member of the Raf family of protein kinasesCell, 1993
- Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 productCell, 1991