Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci
Open Access
- 1 March 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (3), 1141-1144
- https://doi.org/10.1128/jcm.44.3.1141-1144.2006
Abstract
We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus .Keywords
This publication has 26 references indexed in Scilit:
- Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureusJournal of Clinical Microbiology, 2005
- Panton-Valentine Leukocidin: A Marker of Severity for Staphylococcus aureus Infection?Clinical Infectious Diseases, 2005
- Necrotizing Fasciitis Caused by Community-Associated Methicillin-ResistantStaphylococcus aureusin Los AngelesNew England Journal of Medicine, 2005
- Gene sequences and specific detection for Panton-Valentine leukocidinBiochemical and Biophysical Research Communications, 2005
- Emerging Infections with Community-Associated Methicillin-ResistantStaphylococcus aureusin Outpatients at an Army Community HospitalSurgical Infections, 2005
- Rapid detection of Panton–Valentine leukocidin from clinical isolates ofStaphylococcus aureusstrains by real-time PCRFEMS Microbiology Letters, 2004
- Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspectsClinical Microbiology & Infection, 2004
- Molecular Characteristics of Nosocomial and Native American Community-Associated Methicillin-Resistant Staphylococcus aureus Clones from Rural WisconsinJournal of Clinical Microbiology, 2004
- Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patientsThe Lancet, 2002
- Pore formation by a two-component leukocidin from Staphyloccocus aureus within the membrane of human polymorphonuclear leukocytesBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1993