Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites
Open Access
- 17 March 2006
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 20 (7), 820-835
- https://doi.org/10.1101/gad.1391006
Abstract
Dendrite arborization patterns are critical determinants of neuronal function. To explore the basis of transcriptional regulation in dendrite pattern formation, we used RNA interference (RNAi) to screen 730 transcriptional regulators and identified 78 genes involved in patterning the stereotyped dendritic arbors of class I da neurons in Drosophila. Most of these transcriptional regulators affect dendrite morphology without altering the number of class I dendrite arborization (da) neurons and fall primarily into three groups. Group A genes control both primary dendrite extension and lateral branching, hence the overall dendritic field. Nineteen genes within group A act to increase arborization, whereas 20 other genes restrict dendritic coverage. Group B genes appear to balance dendritic outgrowth and branching. Nineteen group B genes function to promote branching rather than outgrowth, and two others have the opposite effects. Finally, 10 group C genes are critical for the routing of the dendritic arbors of individual class I da neurons. Thus, multiple genetic programs operate to calibrate dendritic coverage, to coordinate the elaboration of primary versus secondary branches, and to lay out these dendritic branches in the proper orientation.Keywords
This publication has 54 references indexed in Scilit:
- Development of Morphological Diversity of Dendrites in Drosophila by the BTB-Zinc Finger Protein AbruptNeuron, 2004
- Recent advances in ideas on the molecular pathology and clinical aspects of Von Hippel-Lindau diseaseInternational Journal of Clinical Oncology, 2004
- Olfactory receptor neuron axon targeting: intrinsic transcriptional control and hierarchical interactionsNature Neuroscience, 2004
- Functional Dissection of an Innate Immune Response by a Genome-Wide RNAi ScreenPLoS Biology, 2004
- The Drosophila Brahma (SWI/SNF) chromatin remodeling complex exhibits cell-type specific activation and repression functionsDevelopmental Biology, 2004
- Enhanced Locomotion Caused by Loss of the Drosophila DEG/ENaC Protein Pickpocket1Current Biology, 2003
- Histone Methyltransferase Activity of a Drosophila Polycomb Group Repressor ComplexCell, 2002
- Drosophila Enhancer of Zeste/ESC Complexes Have a Histone H3 Methyltransferase Activity that Marks Chromosomal Polycomb SitesCell, 2002
- Transcriptional Codes and the Control of Neuronal IdentityAnnual Review of Neuroscience, 2002
- Neuronal specification in the spinal cord: inductive signals and transcriptional codesNature Reviews Genetics, 2000