Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling
Open Access
- 1 September 2011
- journal article
- Published by Informa UK Limited in Journal of Inflammation Research
- Vol. 4, 127-138
- https://doi.org/10.2147/jir.s19461
Abstract
Inflammation contributes to the development of fibrotic and malignant diseases. We assessed the ability of inflammatory cytokines to modulate endothelial cell survival and functions related to tissue repair/remodeling. Treatment with interleukin (IL)-1ß or tumor necrosis factor (TNF)-α (2 ng/mL) led to human pulmonary artery endothelial cells becoming spindle-shaped fibroblast-like cells. However, immunoblot and DNA microarray showed no change in most endothelial and mesenchymal markers. In the presence of IL-1ß or TNF-α, cells were resistant to apoptosis induced by deprivation of serum and growth factor, and were more migratory. In addition, cells treated with IL-1ß or TNF-α contracted collagen gels more robustly. In contrast, transforming growth factor-ß1 did not induce these responses. RNA interference targeting nuclear factor (NF)- κB p65 blocked the effects of IL-1ß or TNF-α on cell morphologic change, survival, migration, and collagen gel contraction. These results suggest that endothelial cells may contribute to tissue repair/remodeling via the NF-κB signaling in a milieu of airway inflammation.Keywords
This publication has 20 references indexed in Scilit:
- Endothelial–Mesenchymal Transition in Bleomycin-Induced Pulmonary FibrosisAmerican Journal of Respiratory Cell and Molecular Biology, 2010
- Corneal myofibroblast viability: Opposing effects of IL-1 and TGF β1Experimental Eye Research, 2009
- Inflammatory cytokines augments TGF‐β1‐induced epithelial‐mesenchymal transition in A549 cells by up‐regulating TβR‐ICell Motility, 2008
- NF-κB: a new player in angiostatic therapyAngiogenesis, 2008
- Cultured Lung Fibroblasts from Ovalbumin-Challenged “Asthmatic” Mice Differ Functionally from NormalAmerican Journal of Respiratory Cell and Molecular Biology, 2007
- Perspectives on endothelial-to-mesenchymal transition: potential contribution to vascular remodeling in chronic pulmonary hypertensionAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2007
- Inflammatory cytokines induce the transformation of human dermal microvascular endothelial cells into myofibroblasts: a potential role in skin fibrogenesisJournal of Cutaneous Pathology, 2007
- Induction of Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells by Transforming Growth Factor-β1The American Journal of Pathology, 2005
- Differential Response of Human Lung Epithelial Cells to Fas-Induced ApoptosisThe American Journal of Pathology, 2004
- Interleukin 1 (IL1) and tumour necrosis factor (TNF) signal transductionPhilosophical Transactions Of The Royal Society B-Biological Sciences, 1996