DNA sequencing using a four‐color confocal fluorescence capillary array scanner
- 1 January 1996
- journal article
- instrumentation
- Published by Wiley in Electrophoresis
- Vol. 17 (12), 1852-1859
- https://doi.org/10.1002/elps.1150171209
Abstract
The design, construction and operation of a four-color capillary array electrophoresis scanner are presented. The use of sensitive energy transfer primers facilitates four-color detection of the DNA sequencing fragments following excitation at a single laser wavelength (488 nm). This scanner collects fluorescence data from up to 25 capillaries in parallel. The resulting four-color image files are automatically reduced to four-color line plots, and a base-calling program (Sax) is used to call the sequence. The performance of this system for DNA sequencing is demonstrated by examining twelve different motifs of the hypervariable region I of human mitochondrial (mt) DNA obtained from a Sierra Leone population.Keywords
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