A Convenient Void Volume Marker for Several Chiral HPLC Columns

Abstract

Comparison of a number of potential void volume markers under normal phase conditions shows 1,3,5-Tri-t-butylbenzene (TTBB) to be essentially unretained on three commercially available chiral stationary phases (CSPs). Elution times observed for TTBB agree well with void times determined by the method of minor disturbance, or with the pycnometrically determined total mobile phase volume (Vm). Therefore TTBB is recommended for use as a void volume marker for normal phase separations with these CSPs.