Human liver protein map: A reference database established by microsequencing and gel comparison
- 1 January 1992
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 13 (1), 992-1001
- https://doi.org/10.1002/elps.11501301201
Abstract
This publication establishes a reference human liver protein map obtained with immobilized pH gradients. By microsequencing, 57 spots or 42 polypeptide chains were identified. By protein map comparison and matching (liver, red blood cell and plasma sample maps), 8 additional proteins were identified. The new polypeptides and previously known proteins are listed in a table and/or labeled on the protein map, thus providing a human liver two‐dimensional gel database. This reference map can be used to identify protein spots on other samples such as rectal cancer biopsies.Keywords
This publication has 67 references indexed in Scilit:
- A two‐dimensional gel protein database of noncultured total normal human epidermal keratinocytes: Identification of proteins strongly up‐regulated in psoriatic epidermisElectrophoresis, 1990
- Two‐dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genesElectrophoresis, 1990
- Computerized, comprehensive databases of cellular and secreted proteins from normal human embryonic lung MRC‐5 fibroblasts: Identification of transformation and/or proliferation sensitive proteinsElectrophoresis, 1989
- DirectN-terminal sequence analysis of rat liver plasma membrane glycoproteins separated by two-dimensional polyacrylamide gel electrophoresisElectrophoresis, 1989
- Effects of aroclor 1254 on proteins of mouse liver: Application of two‐dimensional electrophoretic protein mappingElectrophoresis, 1986
- Electrophoretic Analysis of HeLa Cell and Human Liver Nucleolar Proteins and AntigensCancer Investigation, 1985
- Computer analysis of two‐dimensional gels: Automatic matchingElectrophoresis, 1984
- Human liver ribosomal proteins: Characterization by two-dimensional electrophoresis and molecular weight determinationsBiochemical Medicine, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Microheterogeneity of serum transferrin, haptoglobin and α2HS glycoprotein examined by high resolution two-dimensional electrophoresisBiochemical and Biophysical Research Communications, 1979