Combined sacB -Based Negative Selection and cre-lox Antibiotic Marker Recycling for Efficient Gene Deletion in Pseudomonas Aeruginosa
Open Access
- 1 January 2005
- journal article
- research article
- Published by Taylor & Francis Ltd in BioTechniques
- Vol. 38 (1), 63-67
- https://doi.org/10.2144/05381st01
Abstract
The complete genome of the bacterial pathogen Pseudomonas aeruginosa has now been sequenced, allowing gene deletion, one of the most frequently used methods in gene function study, to be fully exploited. In this study, we combine...Funding Information
- the association “Vaincre la Mucoviscidose.”
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