Evidence of Independent Origin of Multiple Tumors From Patients With Prostate Cancer
Open Access
- 4 February 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 90 (3), 233-237
- https://doi.org/10.1093/jnci/90.3.233
Abstract
Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalinfixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.Keywords
This publication has 23 references indexed in Scilit:
- An adjustment to the 1997 estimate for new prostate cancer cases.CA: A Cancer Journal for Clinicians, 1997
- The extent and multicentricity of high-grade prostatic intraepithelial neoplasia in clinically localized prostatic adenocarcinomaHuman Pathology, 1997
- Cancer statistics, 1997CA: A Cancer Journal for Clinicians, 1997
- Age and Racial Distribution of Prostatic Intraepithelial NeoplasiaEuropean Urology, 1996
- Microsatellite alterations as clonal markers for the detection of human cancer.Proceedings of the National Academy of Sciences of the United States of America, 1994
- Clonal Origin of Epithelial Ovarian Carcinoma: Analysis by Loss of Heterozygosity, p53 Mutation, and X-Chromosome InactivationJNCI Journal of the National Cancer Institute, 1992
- A Comparison of the Morphological Features of Cancer Arising in the Transition Zone and in the Peripheral Zone of the ProstateJournal of Urology, 1991
- The Distribution of Residual Cancer in Radical Prostatectomy Specimens in Stage a Prostate CancerJournal of Urology, 1991
- Correlations between Atypical Primary Hyperplasia and Carcinoma of the Prostate: A Histological Study of 180 total ProstatectomiesPathology - Research and Practice, 1980
- Carcinoma of the prostate: Prognostic evaluation of certain pathologic features in 208 radical prostatectomiesCancer, 1972