Changes in Lipogenic Capacity and Activities of Ketolytic and Lipogenic Enzymes in Brain Regions of Developing Rats
- 1 January 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 40 (1), 99-105
- https://doi.org/10.1111/j.1471-4159.1983.tb12658.x
Abstract
Oxidation of ketone bodies (KB) generates acetyl CoA (AcCoA), which can be further incorporated into fatty acid. The rates of lipogenesis from ketone bodies in developing rats and their relation to the activities of enzymes involved in the production of cytoplasmic AcCoA via different pathways in brain regions were determined. In the cerebrum (Cbr), rates of fatty acid synthesis from [3-14C]acetoacetate ([3-14C]AcAc) were high during the early postnatal period but decreased rapidly thereafter until weaning. Although similar developmental patterns of synthesis characterized the cerebellum (Cbl), midbrain (Mb), brain stem (Bs) and thalamus (Th), maximal rates were highest in the Cbr and lowest in the Th. In all regions, synthetic rates were higher throughout the entire suckling period than in adulthood. There were no appreciable differences in synthetic rates among brain regions of adult rats. The developmental changes in rates of AcAc incorporation into fatty acids were closely related to AcAcCoA synthetase activity, but not to activities of ATP-citrate lyase or AcCoA synthetase. During the early postnatal stage the enhanced rates of lipogenesis were accompanied by increased activities of AcAcCoA synthetase in all regions, with the highest activity occurring in the Cbr. The sequence of reactions coupling AcAcCoA synthetase and AcAcCoA thiolase in cytoplasm may be an important pathway for generation of AcCoA from KB for fatty acid synthesis in all regions of the developing brain. This interpretation is strengthened by evidence of concomitant increases in the activities of fatty acid synthetase and AcCoA carboxylase.Keywords
This publication has 32 references indexed in Scilit:
- The Development of Enzymes of Energy Metabolism in the Brain of a Precocial (Guinea Pig) and Non‐Precocial (Rat) SpeciesJournal of Neurochemistry, 1980
- Ketone Bodies Are Selectively Used by Individual Brain RegionsScience, 1979
- Lignoceric Acid Biosynthesis in the Developing Brain. Activities of Mitochondria1 Acetyl‐CoA‐Dependent Synthesis and Microsomal Malonyl‐CoA Chain‐Elongating System in Relation to MyelinationEuropean Journal of Biochemistry, 1977
- Lipids and Development of the Human BrainBiochemical Society Transactions, 1976
- Acetoacetate and brain lipogenesis: developmental pattern of acetoacetyl-coenzyme A synthetase in the soluble fraction of rat brain (Short Communication)Biochemical Journal, 1973
- Metabolism of [1-14c]palmitic acid in the developing brain:Persistence of radioactivity in the carboxyl carbonBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Acetyl CoA Carboxylase and Fatty Acid Synthetase Activities in Liver and Adipose Tissue of Meal-fed RatsExperimental Biology and Medicine, 1969
- The sulfhydryl groups of citrate cleavage enzymeArchives of Biochemistry and Biophysics, 1969
- Liver acetyl CoA carboxylase and fatty acid synthetase: Relative activities in the normal state and in hereditary obesityBiochemical and Biophysical Research Communications, 1967
- Biosynthesis of Fatty AcidsPublished by Elsevier BV ,1960