Sulforaphane Inhibited Melanin Synthesis by Regulating Tyrosinase Gene Expression in B16 Mouse Melanoma Cells
- 23 March 2010
- journal article
- Published by Informa UK Limited in Bioscience, Biotechnology, and Biochemistry
- Vol. 74 (3), 579-582
- https://doi.org/10.1271/bbb.90778
Abstract
Sulforaphane is a compound widely present in consumed vegetables, particularly broccoli. Previous studies have demonstrated that sulforaphane has many physiological effects including anti-cancer, anti-oxidation, and detoxification. In this study, we found that sulforaphane inhibited melanogenesis and tyrosinase expression. The inhibitory effect of 5 μM sulforaphane on melanogenesis was determined to be equivalent to that of 100 μM arbutin. In addition, sulforaphane induced phosphorylated extracellular signal-regulated kinase (ERK) and inhibited phosphorylated p38. It has been reported that the phosphorylated mitogen-activated protein (MAP) kinase family (ERK and p38) controls tyrosinase expression. Our data indicate that sulforaphane inhibited melanogenesis and tyrosinase expression by affecting the phosphorylated MAP kinase family. These findings indicate that sulforaphane might be an effective skin-whitening agent.Keywords
This publication has 23 references indexed in Scilit:
- Potent Induction of Total Cellular and Mitochondrial Antioxidants and Phase 2 Enzymes by Cruciferous Sulforaphane in Rat Aortic Smooth Muscle Cells: Cytoprotection Against Oxidative and Electrophilic StressCardiovascular Toxicology, 2008
- Mechanism of the melanogenesis stimulation activity of (−)-cubebin in murine B16 melanoma cellsBioorganic & Medicinal Chemistry, 2007
- Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the futureCellular and Molecular Life Sciences, 2005
- Inhibitory Effect of an Ellagic Acid-Rich Pomegranate Extract on Tyrosinase Activity and Ultraviolet-Induced PigmentationBioscience, Biotechnology, and Biochemistry, 2005
- Biogenesis of pigment granules: a sensitive way to regulate melanocyte functionJournal of Dermatological Science, 2005
- Gnetol as a Potent Tyrosinase Inhibitor from GenusGnetumBioscience, Biotechnology, and Biochemistry, 2003
- The involvement of p38 mitogen‐activated protein kinase in the α‐melanocyte stimulating hormone (α‐MSH)‐induced melanogenic and anti‐proliferative effects in B16 murine melanoma cellsFEBS Letters, 2000
- Transforming Growth Factor-β1 Inhibits Basal Melanogenesis in B16/F10 Mouse Melanoma Cells by Increasing the Rate of Degradation of Tyrosinase and Tyrosinase-related Protein-1Journal of Biological Chemistry, 1997
- Radical Production During Tyrosinase Reaction, Dopa-Melanin Formation, and Photoirradiation of Dopa-MelaninJournal of Investigative Dermatology, 1984
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976