Vitamin D3 treatment of Crohn’s disease patients increases stimulated T cell IL‐6 production and proliferation
- 23 September 2010
- journal article
- research article
- Published by Wiley in Alimentary Pharmacology & Therapeutics
- Vol. 32 (11-12), 1364-1372
- https://doi.org/10.1111/j.1365-2036.2010.04463.x
Abstract
International audienceIntroduction:Vitamin D3 has shown immune-modulating effects in CD4+ T cells from Crohn's disease (CD) patients in vitro. Aim:To investigate the effects of in vivo vitamin D3 treatment on T cells in CD patients. Methods:Peripheral blood mononuclear cells (PBMC) were isolated at week 0 and week 26 from 10 vitamin D3 and 10 placebo treated CD patients participating in a randomized placebo-controlled clinical trial study. Monocyte-depleted PBMC were stimulated with anti-CD3 and anti-CD28 and cultured for 7 days, to investigate CD4+ T cell proliferation and T cell cytokine production. Results: In vitamin D3 treated patients the median 25-hydroxyvitamin D3 levels increased 70 nmol/l compared to -5 nmol/l in the placebo group. Vitamin D3 treatment increased interleukin-6 production (delta = 188 pg/ml, range: -444 - 4071) compared to a decrease in the placebo group (delta = -896 pg/ml, range: -3841 - 1323) (p < 0.02, Wilcoxon rank sum test). Interestingly vitamin D3 increased the amount of proliferating stimulated CD4+ T cells from median 41 % (range: 10 - 75%), to 56 % (range: 26 - 77%)(p = 0.02, Wilcoxon rank sum test). Conclusions:Vitamin D3 treatment of CD patients increased the IL-6 levels. Interestingly vitamin D3 treatment enhanced the CD4+ T cell proliferationKeywords
This publication has 45 references indexed in Scilit:
- Clinical trial: vitamin D3 treatment in Crohn’s disease – a randomized double‐blind placebo‐controlled studyAlimentary Pharmacology & Therapeutics, 2010
- Late Crohn’s disease patients present an increase in peripheral Th17 cells and cytokine production compared with early patientsAlimentary Pharmacology & Therapeutics, 2010
- Vitamin D Status and Bone Density in Recently Diagnosed Inflammatory Bowel Disease: The Manitoba IBD Cohort StudyThe American Journal of Gastroenterology, 2008
- Inflammatory bowel disease: cause and immunobiologyThe Lancet, 2007
- Seasonality of vitamin D status and bone turnover in patients with Crohn's diseaseAlimentary Pharmacology & Therapeutics, 2005
- IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humansAmerican Journal of Physiology-Endocrinology and Metabolism, 2003
- 1α,25-Dihydroxyvitamin D3 Has a Direct Effect on Naive CD4+ T Cells to Enhance the Development of Th2 CellsThe Journal of Immunology, 2001
- Incidence of inflammatory bowel disease across Europe: is there a difference between north and south? Results of the European Collaborative Study on Inflammatory Bowel Disease (EC-IBD).Gut, 1996
- Seasonal Change in the Exacerbations of Crohn's DiseaseScandinavian Journal of Gastroenterology, 1996
- Altered Th1/Th2 cytokine profiles in the intestinal mucosa of patients with inflammatory bowel disease as assessed by quantitative reversed transcribed polymerase chain reaction (RT-PCR)Clinical and Experimental Immunology, 1995