Oxidative stress sensitivity inDebaryomyces hansenii
Open Access
- 1 June 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Yeast Research
- Vol. 9 (4), 582-590
- https://doi.org/10.1111/j.1567-1364.2009.00500.x
Abstract
Debaryomyces hansenii is an osmotolerant and halotolerant yeast of increasing interest for fundamental and applied research. In this work, we have performed a first study on the effect of oxidative stress on the performance of this yeast. We have used Saccharomyces cerevisiae as a well-known reference yeast. We show that D. hansenii is much more susceptible than S. cerevisiae to cadmium chloride, hydrogen peroxide or 1,4-dithiothreitol. These substances induced the formation of reactive oxygen species (ROS) in both yeasts, the amounts measured being significantly higher in the case of D. hansenii. We also show that NaCl exerted a protective effect against oxidative stress in Debaryomyces, but that this was not the case in Saccharomyces because sodium protected that yeast only when toxicity was induced with cadmium. On the basis of the present results, we raised the hypothesis that the sensitivity to oxidative stress in D. hansenii is related to the high amounts of ROS formed in that yeast and that observations such as low glutathione amounts, low basal superoxide dismutase and peroxidase activities, decrease in ATP levels produced in the presence of ROS inducers and high cadmium accumulation are determinants directly or indirectly involved in the sensitivity process.Keywords
This publication has 25 references indexed in Scilit:
- Vitamin B12b enhances the cytotoxicity of dithiothreitolFree Radical Biology & Medicine, 2008
- The co-action of osmotic and high temperature stresses results in a growth improvement of Debaryomyces hansenii cellsInternational Journal of Food Microbiology, 2007
- Oxidative stress in Schizosaccharomyces pombe: different H2O2 levels, different response pathwaysMolecular Genetics and Genomics, 2006
- Membrane transport of hydrogen peroxideBiochimica et Biophysica Acta (BBA) - Biomembranes, 2006
- Tomato QM-Like Protein Protects Saccharomyces cerevisiae Cells against Oxidative Stress by Regulating Intracellular Proline LevelsApplied and Environmental Microbiology, 2006
- Genome evolution in yeastsNature, 2004
- Cloning and Expression of Two Genes Coding for Sodium Pumps in the Salt-Tolerant Yeast Debaryomyces hanseniiJournal of Bacteriology, 2001
- A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: performance study of a new miniaturized protocolCell Biology and Toxicology, 1994
- Regulation of potassium fluxes in Saccharomyces cerevisiaeBiochimica et Biophysica Acta (BBA) - Biomembranes, 1990
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976