Energy Conservation in the Acetogenic Bacterium Clostridium aceticum
Open Access
- 27 January 2021
- journal article
- research article
- Published by MDPI AG in Microorganisms
- Vol. 9 (2), 258
- https://doi.org/10.3390/microorganisms9020258
Abstract
In times of global warming caused by the extensive use of fossil fuels, the need to capture gaseous carbon compounds is growing bigger. Several groups of microorganisms can fix the greenhouse gas CO2. Out of these, acetogenic bacteria are role models in their ability to reduce CO2 with hydrogen to acetate, which makes acetogens prime candidates for genetic modification towards biotechnological production of value-added compounds from CO2, such as biofuels. However, growth of acetogens on gaseous substrates is strongly energy-limited, and successful metabolic engineering requires a detailed knowledge of the bioenergetics. In 1939, Clostridium aceticum was the first acetogen to be described. A recent genomic study revealed that this organism contains cytochromes and therefore may use a proton gradient in its respiratory chain. We have followed up these studies and will present data that C. aceticum does not use a H+ but a Na+ gradient for ATP synthesis, established by a Na+-Rnf. Experimental data and in silico analyses enabled us to propose the biochemistry and bioenergetics of acetogenesis from H2 + CO2 in C. aceticum.Keywords
Funding Information
- BMBF (Era-IB OBAC, grant number ERA-IB-16–018)
This publication has 47 references indexed in Scilit:
- Clostridium acidurici Electron-Bifurcating Formate DehydrogenaseApplied and Environmental Microbiology, 2013
- A Bacterial Electron-bifurcating HydrogenaseOnline Journal of Public Health Informatics, 2012
- Promiscuous archaeal ATP synthase concurrently coupled to Na + and H + translocationProceedings of the National Academy of Sciences of the United States of America, 2012
- Biochemistry, evolution and physiological function of the Rnf complex, a novel ion-motive electron transport complex in prokaryotesCellular and Molecular Life Sciences, 2010
- Bacterial Na + -translocating ferredoxin:NAD + oxidoreductaseProceedings of the National Academy of Sciences of the United States of America, 2010
- Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixationBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2008
- The complete genome sequence ofMoorella thermoacetica(f.Clostridium thermoaceticum)Environmental Microbiology, 2008
- Enzymology of the Wood–Ljungdahl Pathway of AcetogenesisAnnals of the New York Academy of Sciences, 2008
- Sodium ion dependence of inhibition of the Na+-translocating F1F0-ATPase from Acetobacterium woodii. Probing the site(s) involved in ion transportBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1995
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976