Reduced expression of a subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1βγ, confers flat fruit abortion in peach by regulating sugar and starch metabolism
Open Access
- 10 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Plant Biology
- Vol. 21 (1), 1-13
- https://doi.org/10.1186/s12870-021-02850-9
Abstract
Fruit abortion is a major limiting factor for fruit production. In flat peach, fruit abortion is present in the whole tree of some accessions during early fruit development. However, the physiological factors and genetic mechanism underlying flat fruit abortion remain largely elusive. In this study, we have revealed that the fertilization process was accomplished and the reduction of sucrose and starch contents might result in flat fruit abortion. By combining association and gene expression analysis, a key candidate gene, PpSnRK1βγ, was identified. A 1.67-Mb inversion co-segregated with flat fruit shape altered the promoter activity of PpSnRK1βγ, resulting in much lower expression in aborting flat peach. Ectopic transformation in tomato and transient overexpression in peach fruit have shown that PpSnRK1βγ could increase sugar and starch contents. Comparative transcriptome analysis further confirmed that PpSnRK1βγ participated in carbohydrate metabolism. Subcellular localization found that PpSnRK1βγ was located in nucleus. This study provides a possible reason for flat fruit abortion and identified a critical candidate gene, PpSnRK1βγ, that might be responsible for flat fruit abortion in peach. The results will provide great help in peach breeding and facilitate gene identification for fruit abortion in other plant species.Keywords
Funding Information
- National Natural Science Foundation of China (31972392)
This publication has 57 references indexed in Scilit:
- Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and CufflinksNature Protocols, 2012
- Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and TransportPlant and Cell Physiology, 2010
- BLAST+: architecture and applicationsBMC Bioinformatics, 2009
- Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stressProceedings of the National Academy of Sciences of the United States of America, 2009
- RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and FertilityPlant Physiology, 2009
- Multiple Models for Rosaceae GenomicsPlant Physiology, 2008
- AMP-activated/SNF1 protein kinases: conserved guardians of cellular energyNature Reviews Molecular Cell Biology, 2007
- A central integrator of transcription networks in plant stress and energy signallingNature, 2007
- SNF1/AMPK/SnRK1 kinases, global regulators at the heart of energy control?Trends in Plant Science, 2007
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001