Gelatin Methacryloyl Bioadhesive Improves Survival and Reduces Scar Burden in a Mouse Model of Myocardial Infarction
Open Access
- 2 June 2020
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of the American Heart Association
- Vol. 9 (11), e014199
- https://doi.org/10.1161/JAHA.119.014199
Abstract
BACKGROUND: Delivery of hydrogels to the heart is a promising strategy for mitigating the detrimental impact of myocardial infarction (MI). Challenges associated with the in vivo delivery of currently available hydrogels have limited clinical translation of this technology. Gelatin methacryloyl (GelMA) bioadhesive hydrogel could address many of the limitations of available hydrogels. The goal of this proof-of-concept study was to evaluate the cardioprotective potential of GelMA in a mouse model of MI. METHODS AND RESULTS: The physical properties of GelMA bioadhesive hydrogel were optimized in vitro. Impact of GelMA bioadhesive hydrogel on post-MI recovery was then assessed in vivo. In 20 mice, GelMA bioadhesive hydrogel was applied to the epicardial surface of the heart at the time of experimental MI. An additional 20 mice underwent MI but received no GelMA bioadhesive hydrogel. Survival rates were compared for GelMA-treated and untreated mice. Left ventricular function was assessed 3 weeks after experimental MI with transthoracic echocardiography. Left ventricular scar burden was measured with postmortem morphometric analysis. Survival rates at 3 weeks post-MI were 89% for GelMA-treated mice and 50% for untreated mice (P=0.011). Left ventricular contractile function was better in GelMA-treated than untreated mice (fractional shortening 37% versus 26%, P<0.001). Average scar burden in GelMA-treated mice was lower than in untreated mice (6% versus 22%, P=0.017). CONCLUSIONS: Epicardial GelMA bioadhesive application at the time of experimental MI was performed safely and was associated with significantly improved post-MI survival compared with control animals. In addition, GelMA treatment was associated with significantly better preservation of left ventricular function and reduced scar burden.This publication has 60 references indexed in Scilit:
- Design Strategies and Applications of Tissue BioadhesivesMacromolecular Bioscience, 2012
- Catheter-Deliverable Hydrogel Derived From Decellularized Ventricular Extracellular Matrix Increases Endogenous Cardiomyocytes and Preserves Cardiac Function Post-Myocardial InfarctionJournal of the American College of Cardiology, 2012
- Low-density lipoprotein receptor gene transfer in hypercholesterolemic mice improves cardiac function after myocardial infarctionGene Therapy, 2011
- Increased Infarct Wall Thickness by a Bio-Inert Material Is Insufficient to Prevent Negative Left Ventricular Remodeling after Myocardial InfarctionPLOS ONE, 2011
- Controlled gelation and degradation rates of injectable hyaluronic acid-based hydrogels through a double crosslinking strategyJournal of Tissue Engineering and Regenerative Medicine, 2011
- THE AESTHETIC APPLICATIONS OF INTENSE PULSED LIGHT USING THE LUMENIS M-22 DEVICELASER THERAPY, 2011
- Epidemiology and risk profile of heart failureNature Reviews Cardiology, 2010
- Controlling the Porosity and Microarchitecture of Hydrogels for Tissue EngineeringTissue Engineering, Part B: Reviews, 2010
- Cell-laden microengineered gelatin methacrylate hydrogelsBiomaterials, 2010
- Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approachesJournal of Applied Physiology, 2007