Metallo-beta-lactamase-producing Escherichia coli in the sewage of Mexico City: where do they come from?
- 18 October 2021
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 68 (2), 1-7
- https://doi.org/10.1139/cjm-2021-0284
Abstract
While monitoring the presence of antibiotic resistance in municipal wastewater bacteria from Mexico City, five Escherichia coli isolates were detected to be resistant to carbapenems, antibiotics of “last resort” used mostly in hospitals. Further analysis revealed that these carbapenem-resistant isolates carried the gene for a metallo-beta-lactamase, NDM-5. The gene was found to be beared by a large, ~145 kb conjugative plasmid, which also carries putative genes encoding resistance to sulfonamides, trimethoprim, tetracycline, ciprofloxacin, chloramphenicol (although no phenotypic chloramphenicol resistance was detected) and quaternary-ammonium compounds. The plasmid also carried gene mobility determinants, such as an integron integrase, and two transposases. In addition to the direct public health threat posed by the presence of such multi-resistant organisms in wastewater released into the environment and used for crop irrigation; it is particularly concerning that carbapenem-resistant E. coli is rather rare in Mexican hospitals (<1%), but was found in small, 100-mL samples of municipal wastewater. This could suggest that, either these organisms are under-reported by clinical microbiology laboratories, underlining the usefulness of wastewater monitoring; or that there is an unknown source of such carbapenem-resistant organisms that are being dumped into the wastewater. The source of these bacteria must be assessed and controlled to prevent the further spread of this multi-resistance plasmid among other environmental and clinical microorganisms.Keywords
This publication has 39 references indexed in Scilit:
- Antibiotic Resistance: From Darwin to Lederberg to KeynesMicrobial Drug Resistance, 2013
- Origin and Evolution of Antibiotic Resistance: The Common Mechanisms of Emergence and Spread in Water BodiesFrontiers in Microbiology, 2012
- A Novel Variant, NDM-5, of the New Delhi Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli ST648 Isolate Recovered from a Patient in the United KingdomAntimicrobial Agents and Chemotherapy, 2011
- Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological studyThe Lancet Infectious Diseases, 2010
- genoPlotR: comparative gene and genome visualization in RBioinformatics, 2010
- Measuring dementia carers' unmet need for services - an exploratory mixed method studyBMC Health Services Research, 2010
- Fluoroquinolone resistance in clinical and environmental isolates of Escherichia coli in Mexico CityJournal of Applied Microbiology, 2010
- An analysis of the evolutionary relationships of integron integrases, with emphasis on the prevalence of class 1 integrons in Escherichia coli isolates from clinical and environmental originsMicrobiology, 2008
- Conjugation in Escherichia coliBiochemistry and Molecular Biology Education, 2007
- Imipenem-EDTA Disk Method for Differentiation of Metallo-β-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter sppJournal of Clinical Microbiology, 2002