Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors
Open Access
- 22 May 2020
- journal article
- research article
- Published by Oxford University Press (OUP) in Stem Cells Translational Medicine
- Vol. 9 (9), 1036-1052
- https://doi.org/10.1002/sctm.19-0406
Abstract
Human induced pluripotent stem cells (hiPSCs) have generated a great deal of attention owing to their capacity for self‐renewal and differentiation into the three germ layers of the body. Their discovery has facilitated a new era in biomedicine for understanding human development, drug screening, disease modeling, and cell therapy while reducing ethical issues and risks of immune rejection associated with traditional embryonic stem cells. Bioreactor‐based processes have been the method of choice for the efficient expansion and differentiation of stem cells in controlled environments. Current protocols for the expansion of hiPSCs use horizontal impeller, paddle, or rocking wave mixing method bioreactors which require large static cell culture starting populations and achieve only moderate cell fold increases. This study focused on optimizing inoculation, agitation, oxygen, and nutrient availability for the culture of hiPSCs as aggregates in single‐use, low‐shear, vertical‐wheel bioreactors. Under optimized conditions, we achieved an expansion of more than 30‐fold in 6 days using a small starting population of cells and minimal media resources throughout. Importantly, we showed that that this optimized bioreactor expansion protocol could be replicated over four serial passages resulting in a cumulative cell expansion of 1.06E6‐fold in 28 days. Cells from the final day of the serial passage were of high quality, maintaining a normal karyotype, pluripotent marker staining, and the ability to form teratomas in vivo. These findings demonstrate that a vertical‐wheel bioreactor‐based bioprocess can provide optimal conditions for efficient, rapid generation of high‐quality hiPSCs to meet the demands for clinical manufacturing of therapeutic cell products.Keywords
Funding Information
- Alberta Children's Hospital Research Institute Clinical Research Fellowship
- Vanier Canada Graduate Scholarship program
- Collaborative Health Research Projects
- NSERC Discovery Grant Program
- Alberta Children's Hospital Research Institute Clinical Research Fellowship
- Vanier Canada Graduate Scholarship program
- Collaborative Health Research Projects
- NSERC Discovery Grant Program
This publication has 44 references indexed in Scilit:
- Induced pluripotent stem cells in medicine and biologyDevelopment, 2013
- Process engineering of human pluripotent stem cells for clinical applicationTrends in Biotechnology, 2012
- Stem cell cultivation in bioreactorsBiotechnology Advances, 2011
- Expansion and long-term maintenance of induced pluripotent stem cells in stirred suspension bioreactorsJournal of Tissue Engineering and Regenerative Medicine, 2011
- Scalable expansion of human pluripotent stem cells in suspension cultureNature Protocols, 2011
- Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to PluripotencyCell Stem Cell, 2011
- Derivation, propagation and controlled differentiation of human embryonic stem cells in suspensionNature Biotechnology, 2010
- Embryonic stem cells remain highly pluripotent following long term expansion as aggregates in suspension bioreactorsJournal of Biotechnology, 2007
- Expansion of Undifferentiated Murine Embryonic Stem Cells as Aggregates in Suspension Culture BioreactorsTissue Engineering, 2006
- Derivation of human embryonic stem cells in defined conditionsNature Biotechnology, 2006