The protein kinase Akt acts as a coat adaptor in endocytic recycling
- 15 June 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 22 (8), 927-933
- https://doi.org/10.1038/s41556-020-0530-z
Abstract
Coat proteins have a central role in vesicular transport by binding to cargoes for their sorting into intracellular pathways. Cargo recognition is mediated by components of the coat complex known as adaptor proteins1,2,3. We previously showed that Arf-GAP with coil-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) functions as an adaptor for a clathrin coat complex that has a function in endocytic recycling4,5,6. Here, we show that the protein kinase Akt acts as a co-adaptor in this complex, and is needed in conjunction with ACAP1 to bind to cargo proteins to promote their recycling. In addition to advancing the understanding of endocytic recycling, we uncover a fundamentally different function in which a kinase acts, as Akt in this case is an effector rather than a regulator in a cellular event.This publication has 30 references indexed in Scilit:
- ERK1/2 Regulate Exocytosis through Direct Phosphorylation of the Exocyst Component Exo70Developmental Cell, 2012
- Regulation of coat assembly—sorting things out at the ERCurrent Opinion in Cell Biology, 2010
- Differential Effects of Cytohesins 2 and 3 on β1 Integrin RecyclingOnline Journal of Public Health Informatics, 2010
- Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2Proceedings of the National Academy of Sciences of the United States of America, 2010
- Pathways and mechanisms of endocytic recyclingNature Reviews Molecular Cell Biology, 2009
- The closure of Pak1-dependent macropinosomes requires the phosphorylation of CtBP1/BARSThe EMBO Journal, 2008
- An ACAP1-containing clathrin coat complex for endocytic recyclingThe Journal of cell biology, 2007
- Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1γProceedings of the National Academy of Sciences of the United States of America, 2007
- Adaptable adaptors for coated vesiclesTrends in Cell Biology, 2004
- Signals for Sorting of Transmembrane Proteins to Endosomes and LysosomesAnnual Review of Biochemistry, 2003