A novel d-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae
Open Access
- 26 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-12
- https://doi.org/10.1038/s41598-021-83937-z
Abstract
Carbohydrate rich substrates such as lignocellulosic hydrolysates remain one of the primary sources of potentially renewable fuel and bulk chemicals. The pentose sugar d-xylose is often present in significant amounts along with hexoses. Saccharomyces cerevisiae can acquire the ability to metabolize d-xylose through expression of heterologous d-xylose isomerase (XI). This enzyme is notoriously difficult to express in S. cerevisiae and only fourteen XIs have been reported to be active so far. We cloned a new d-xylose isomerase derived from microorganisms in the gut of the wood-feeding beetle Odontotaenius disjunctus. Although somewhat homologous to the XI from Piromyces sp. E2, the new gene was identified as bacterial in origin and the host as a Parabacteroides sp. Expression of the new XI in S. cerevisiae resulted in faster aerobic growth than the XI from Piromyces on d-xylose media. The d-xylose isomerization rate conferred by the new XI was also 72% higher, while absolute xylitol production was identical in both strains. Interestingly, increasing concentrations of xylitol (up to 8 g L−1) appeared not to inhibit d-xylose consumption. The newly described XI displayed 2.6 times higher specific activity, 37% lower KM for d-xylose, and exhibited higher activity over a broader temperature range, retaining 51% of maximal activity at 30 °C compared with only 29% activity for the Piromyces XI.This publication has 70 references indexed in Scilit:
- Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24Biotechnology for Biofuels, 2013
- Co-fermentation of xylose and cellobiose by an engineered Saccharomyces cerevisiaeJournal of Industrial Microbiology & Biotechnology, 2012
- Directed Evolution of Xylose Isomerase for Improved Xylose Catabolism and Fermentation in the Yeast Saccharomyces cerevisiaeApplied and Environmental Microbiology, 2012
- Environmental and Gut Bacteroidetes: The Food ConnectionFrontiers in Microbiology, 2011
- Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic libraryBiotechnology for Biofuels, 2011
- New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0Systematic Biology, 2010
- Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiaeApplied and Environmental Microbiology, 2009
- Enzymatic deconstruction of xylan for biofuel productionGCB Bioenergy, 2009
- A Probabilistic Model of Local Sequence Alignment That Simplifies Statistical Significance EstimationPLoS Computational Biology, 2008
- The beetle gut: a hyperdiverse source of novel yeastsMycological Research, 2005