Evaluation of different analytical methods to assess failure of passive transfer in neonatal calves
- 1 June 2020
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 103 (6), 5387-5397
- https://doi.org/10.3168/jds.2019-17928
Abstract
The objective of this study was to evaluate different analytical methods of assessing failure of passive transfer (FPT) in neonatal calves. We hypothesized that 3 different media (i.e., centrifuged serum, centrifuged plasma, filtered plasma) and different analytical methods [i.e., ELISA, capillary electrophoresis (CE), Brix refractometer, and handheld optical refractometer] would be highly correlated with the gold standard radial immunodiffusion (RID) and would generate comparable results. Serum and plasma blood samples were collected from Holstein Friesian calves (n = 216) aged 1 to 7 d, from 2 commercial dairy herds in northeast Germany. The RID analysis showed that 59 of 216 calves (27%) had serum IgG concentrations of 10 mg/mL. The mean IgG concentration (+/- standard deviation) was 17.1 +/- 9.8 mg/mL, and the range was 0.8 to 47.8 mg/mL. In serum, the correlation between RID and CE was r = 0.97, and between RID and ELISA was r = 0.90; CE and ELISA were also highly correlated (r = 0.89). Both refractometry methods were highly correlated with RID using centrifuged serum, centrifuged plasma, or filtered plasma (Brix refractometer: r = 0.84, 0.80, and 0.78, respectively; handheld optical refractometer: r = 0.83, 0.81, and 0.80, respectively). We determined test characteristics (optimum thresholds, sensitivity, specificity, positive predictive value, negative predictive value, and area under the curve) for CE, ELISA, and the handheld optical and digital refractometers using receiver operating characteristic curve analyses with RID as the reference value. Optimal thresholds for assessing FPT using plasma were higher than for serum, regardless of the method of plasma harvesting. The 4 different devices had comparable areas under the curve, irrespective of the medium used. All analytical methods can be used to assess FPT.Keywords
This publication has 47 references indexed in Scilit:
- LACTATION BIOLOGY SYMPOSIUM: The effect of nutrient intake from milk or milk replacer of preweaned dairy calves on lactation milk yield as adults: A meta-analysis of current data1Journal of Animal Science, 2013
- Protein separation by capillary gel electrophoresis: A reviewAnalytica Chimica Acta, 2012
- Colostrum Management for Dairy CalvesVeterinary Clinics of North America: Food Animal Practice, 2008
- Enzyme‐Linked Immunosorbent Assay, Single Radial Immunodiffusion, and Indirect Methods for the Detection of Failure of Transfer of Passive Immunity in Dairy CalvesJournal of Veterinary Internal Medicine, 2008
- Vergleich von MessmethodenDeutsche Medizinische Wochenschrift (1946), 2007
- Managing the production, storage, and delivery of colostrumVeterinary Clinics of North America: Food Animal Practice, 2004
- Applying the right statistics: analyses of measurement studiesUltrasound in Obstetrics & Gynecology, 2003
- Evaluation of 3 Assays for Failure of Passive Transfer in CalvesJournal of Veterinary Internal Medicine, 1996
- Analysis of bovine immunoglobulin G by capillary gel electrophoresisJournal of Pharmaceutical and Biomedical Analysis, 1994
- Measuring the Accuracy of Diagnostic SystemsScience, 1988