MAC5, an RNA-binding protein, protects pri-miRNAs from SERRATE-dependent exoribonuclease activities
- 22 September 2020
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 117 (38), 23982-23990
- https://doi.org/10.1073/pnas.2008283117
Abstract
MAC5 is a component of the conserved MOS4-associated complex. It plays critical roles in development and immunity. Here we report that MAC5 is required for microRNA (miRNA) biogenesis. MAC5 interacts with Serrate (SE), which is a core component of the microprocessor that processes primary miRNA transcripts (pri-miRNAs) into miRNAs and binds the stem-loop region of pri-miRNAs. MAC5 is essential for both the efficient processing and the stability of primiRNAs. Interestingly, the reduction of pri-miRNA levels in macs is partially caused by XRN2/XRN3, the nuclear-localized 5'-to-3' exoribonucleases, and depends on SE. These results reveal that MAC5 plays a dual role in promoting pri-miRNA processing and stability through its interaction with SE and/or pri-miRNAs. This study also uncovers that pri-miRNAs need to be protected from nuclear RNA decay machinery, which is connected to the microprocessor.Funding Information
- National Science Foundation of China (31872816)
- the National Institutes of Health (GM127414)
- National Science Foundation (MCB-1808182)
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