Distinct Pattern of NPY in Gastro–Entero–Pancreatic System of Goat Kids Fed with a New Standardized Red Orange and Lemon Extract (RLE)
Open Access
- 9 February 2021
- Vol. 11 (2), 449
- https://doi.org/10.3390/ani11020449
Abstract
The use of natural compounds as feed additive is also increasing in farm animals, thanks to the beneficial effect on both animals and consumers health. Here, we questioned whether natural extracts, such as red orange and lemon extract (RLE) rich in flavanones, anthocyanins, and other polyphenols, used as feed additives could display an effect on the neuropeptide Y (NPY) in the gastro–entero–pancreatic tract of goat kids. NPY is one of the most abundant neuropeptides in mammals, known for its orexigenic role although it is involved in many central and peripheral functions. We carried out immunohistochemical analyses on samples of abomasum, duodenum and pancreas collected from two experimental groups: one fed with standard diet and one with standard diet + RLE. For the first time we document NPY distribution in the abomasum, duodenum and pancreas of goats and observe the highest number of NPY positive cells in neuroendocrine cells of duodenum. Remarkably, upon RLE feed supplementation, NPY immunoreactive cells increased significantly in abomasal epithelium and pancreatic islets but not in duodenum, likely due to pH variation of abomasum and duodenum. Our observations represent a baseline for future studies on the interaction between neuropeptides and polyphenols, used as feed additive.Keywords
This publication has 67 references indexed in Scilit:
- Inhibition of Carrageenan-Induced Acute Inflammation in Mice by Oral Administration of Anthocyanin Mixture from Wild Mulberry and Cyanidin-3-GlucosideBioMed Research International, 2013
- Pancreatic Beta Cells Synthesize Neuropeptide Y and Can Rapidly Release Peptide Co-TransmittersPLOS ONE, 2011
- Pancreatic β Cells Require NeuroD to Achieve and Maintain Functional MaturityCell Metabolism, 2010
- Natural bioactive compounds of Citrus limon for food and healthJournal of Pharmaceutical and Biomedical Analysis, 2010
- 5‐Hydroxytryptamine contributes significantly to a reflex pathway by which the duodenal mucosa protects itself from gastric acid injuryThe FASEB Journal, 2006
- Neuroimmune Link in the Mucosa of Chronic Gastritis with Helicobacter pylori InfectionDigestive Diseases and Sciences, 2006
- The jejunum is the main site of absorption for anthocyanins in miceThe Journal of Nutritional Biochemistry, 2006
- Citrus limon: a source of flavonoids of pharmaceutical interestFood Chemistry, 2004
- Isolation of C-Glucosylflavone from Lemon Peel and Antioxidative Activity of Flavonoid Compounds in Lemon FruitJournal of Agricultural and Food Chemistry, 1997
- PYY in developing murine islet cells: comparisons to development of islet hormones, NPY, and BrdU incorporation.Journal of Histochemistry & Cytochemistry, 1996