Cytotoxicity and mutagenicity of the waters of the Marrecas River (Paraná, Brazil) to bullfrogs (Lithobates catesbeianus)
- 7 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Environmental Science and Pollution Research
- Vol. 28 (17), 21742-21753
- https://doi.org/10.1007/s11356-020-12026-x
Abstract
Animals have a long history of assessing ecosystem responses to environmental disturbances, and amphibians stand out for presenting themselves as good animal model and bioindicators of environmental quality. The main purpose of the present work was to investigate the cellular effects of contamination of waters of the Marrecas River, located in the southwest of the state of Paraná, Brazil. Therefore, the objective of the present study was to investigate and monitor the cytotoxic and mutagenic effect, with bullfrog tadpoles, and to discuss these effects with land use along this hydrographic basin. Mutagenic effects were determined by micronucleus assay, and cytotoxicity by other nuclear changes, such as segmented cells, binucleated cells, cells with buds and reniform cells. Water samples were obtained at nine sites along the Marrecas River, covering areas with rural and urban hydrological contribution. For each site, four samples were collected, along the years 2017 and 2018, encompassing the four seasons (summer, autumn, winter, and spring). The results showed mutagenic and cytotoxic effect in four sampling sites, and only cytotoxic effect in other four sites. These effects may be due, possibly, to the use of different agrochemicals across the hydrographic basin region, which have predominant hydrological contributions from crops. Data of this study indicate the presence of cytotoxic and mutagenic contaminants in the waters of the Marrecas River, which can generate environmental problems on the river fauna/flora, and can also affect the local population health.Keywords
This publication has 45 references indexed in Scilit:
- Use of Lithobates catesbeianus Tadpoles in a Multiple Biomarker Approach for the Assessment of Water Quality of the Reconquista River (Argentina)Archives of Environmental Contamination and Toxicology, 2013
- Widespread Occurrence of the American Bullfrog,Lithobates catesbeianus(Shaw, 1802) (Anura: Ranidae), in BrazilSouth American Journal of Herpetology, 2011
- A cocktail of contaminants: how mixtures of pesticides at low concentrations affect aquatic communitiesOecologia, 2008
- Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifluralin herbicidePesticide Biochemistry and Physiology, 2007
- Cytokinesis-block micronucleus cytome assayNature Protocols, 2007
- Induction of micronuclei in tadpoles ofOdontophrynus americanus(Amphibia: Leptodactylidae) by the pyrethroid insecticide cypermethrinToxicological & Environmental Chemistry, 2006
- Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluentsAquatic Toxicology, 2005
- Conservation genetics of amphibiansHeredity, 2005
- Biomonitoring of the genotoxic potential (micronucleus assay) and detoxifying activity (EROD induction) in the River Dadou (France), using the amphibian Xenopus laevisScience of The Total Environment, 2004
- Micronuclei induction in Rana catesbeiana tadpoles by the pyrethroid insecticide lambda-cyhalothrinGenetics and Molecular Biology, 2003