Postzygotic reproductive isolation among three Saccharomyces yeast species
- 14 January 2021
- Vol. 38 (5), 326-335
- https://doi.org/10.1002/yea.3549
Abstract
We have previously isolated heterothallic haploid strains from original homothallic diploids of two yeast species in the family Saccharomycetaceae. In this study, heterothallic haploid strains were isolated from an original homothallic diploid of Saccharomyces kudriavzevii type strain, followed by investigation of sexual interactions among these yeast strains, in addition to S. cerevisiae laboratory strains. It has been shown that prezygotic reproductive isolation was observed between Kazachstania naganishii and S. cerevisiae with α‐factor mating pheromones representing crossaction with each other beyond the genus boundary. Using heterothallic strains, postzygotic reproductive isolation system was shown to reside in the genus Saccharomyces by mass mating and cell‐cell contact experiments. In mass mating experiments, crossaction of α‐factor and a‐factor mating pheromones and sexual agglutination effectively occurred beyond species boundaries among S. kudriavzevii, S. paradoxus, and S. cerevisiae. When the fates of cell‐cell pairs from these Saccharomyces yeast species were systematically chased one by one, interspecific F1 hybrids were effectively produced, while sporulations were partially prohibited, with spore germination perfectly blocked in the hybrids. These results indicated that postzygotic reproductive isolation definitively resides among these Saccharomyces yeast species and that disorder of chromosome organization had to some extent occurred in interspecific F1 hybrids.Keywords
Funding Information
- Ministry of Education, Culture, Sports, Science and Technology (12640688)
This publication has 30 references indexed in Scilit:
- Characterization of Chromosome Stability in Diploid, Polyploid and Hybrid Yeast CellsPLOS ONE, 2013
- Reconstruction of the Evolutionary History of Saccharomyces cerevisiae x S. kudriavzevii Hybrids Based on Multilocus Sequence AnalysisPLOS ONE, 2012
- Molecular genetic diversity of the Saccharomyces yeasts in Taiwan: Saccharomyces arboricola, Saccharomyces cerevisiae and Saccharomyces kudriavzeviiAntonie van Leeuwenhoek, 2012
- Comparative genomics among Saccharomyces cerevisiae × Saccharomyces kudriavzevii natural hybrid strains isolated from wine and beer reveals different originsBMC Genomics, 2012
- Microbe domestication and the identification of the wild genetic stock of lager-brewing yeastProceedings of the National Academy of Sciences of the United States of America, 2011
- Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzeviiApplied and Environmental Microbiology, 2009
- Molecular Characterization of New Natural Hybrids of Saccharomyces cerevisiae and S . kudriavzevii in BrewingApplied and Environmental Microbiology, 2008
- Natural Populations of Saccharomyces kudriavzevii in Portugal Are Associated with Oak Bark and Are Sympatric with S. cerevisiae and S. paradoxusApplied and Environmental Microbiology, 2008
- Sequence Diversity, Reproductive Isolation and Species Concepts in SaccharomycesGenetics, 2006
- Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of Saccharomyces exiguusFEBS Letters, 1986