The KLF14 Variant is Associated with Type 2 Diabetes and HbA1C Level
- 3 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biochemical Genetics
- Vol. 59 (2), 574-588
- https://doi.org/10.1007/s10528-020-10015-w
Abstract
The purpose of this study was to scan variants in coding region of Krȕppel like factor14 (KLF14) locus and assess association related to type 2 diabetes (T2D) in Iranian population. We sequenced the coding region of KLF14 to scan variants in case–sibling study (92 individuals with T2D and 92 healthy older siblings). To confirm, we analyzed rs76603546 association with T2D in a larger unrelated case–control study by PCR–RFLP (475 cases and 512 controls). We analyzed the association of rs76603546 with HbA1C, BMI, fat mass, waist circumference, fasting glucose, cholesterol and HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) using one-way ANOVA analysis. Also, association of genotypes with T2D adjusted for confounding variables was analyzed using logistic regression. HaploReg v 4.1 was used to predict rs76603546 possible function. Sequencing results analysis revealed the association of C allele of rs76603546, synonymous variant C>T, [OR 2.10 (1.38–3.20), P value < 0.001] and CC genotype of rs76603546 [OR 4.3 (1.79–10.23), P value = 0.001] with susceptibility to T2D. PCR-Restriction Fragment Length Polymorphism (RFLP) results analysis confirmed the association of rs76603546 with T2D [C allele, OR 1.91 (1.59–2.29), P value = 0.002, CC genotype, OR 3.27 (2.26–4.73), P value = 0.002 and TC genotype, OR 1.74 (1.31–2.31), P value = 0.001]. The CC genotype of rs76603546 is associated with HbA1C level (P value < 0.001) and BMI (P value = 0.02). After adjustment with confounding variables, we observed association of CC genotype with T2D [OR 2.542 (1.25–3.77), P value = 0.03]. Among over 220 SNPs, rs76603546 was associated with T2D, HbA1C and BMI in our study.Keywords
Funding Information
- ROJETechnologies (R017-3251/2015)
- Shahid Sadoughi University of Medical Sciences Research Council (268471)
This publication has 41 references indexed in Scilit:
- Plasma Sphingosine-1-Phosphate Is Elevated in ObesityPLOS ONE, 2013
- Association of theKLF14rs4731702 SNP and Serum Lipid Levels in the Guangxi Mulao and Han PopulationsBioMed Research International, 2013
- Chapter 11: Genome-Wide Association StudiesPLoS Computational Biology, 2012
- Waist circumference a good indicator of future risk for type 2 diabetes and cardiovascular diseaseBMC Public Health, 2012
- Identification of an imprinted master trans regulator at the KLF14 locus related to multiple metabolic phenotypesNature Genetics, 2011
- Twelve type 2 diabetes susceptibility loci identified through large-scale association analysisNature Genetics, 2010
- Silencing of the Transforming Growth Factor-β (TGFβ) Receptor II by Krüppel-like Factor 14 Underscores the Importance of a Negative Feedback Mechanism in TGFβ SignalingJournal of Biological Chemistry, 2009
- Gene Set Enrichment in eQTL Data Identifies Novel Annotations and Pathway RegulatorsPLoS Genetics, 2008
- Identification of the Imprinted KLF14 Transcription Factor Undergoing Human-Specific Accelerated EvolutionPLoS Genetics, 2007
- Specifie codon usage pattern and its implications on the secondary structure of silk fibroin mRNAJournal of Molecular Biology, 1988